Department of Microbiology, Theodor Bilharz Research Institute, Giza, Egypt.
APMIS. 2010 Jan;118(1):37-44. doi: 10.1111/j.1600-0463.2009.02556.x.
The present study was designed to investigate the prevalence of herpes simplex virus type-2 (HSV-2) in Egyptian patients with bladder cancer or cystitis and to evaluate the performance of different diagnostic HSV-2 assays. The study included 50 patients: 27 with bladder cancer (group I), 23 with cystitis (group II) and 20 subjects as controls (group III). HSV-2 DNA was detected using polymerase chain reaction (PCR) on bladder tissue and buffy coat cells (BCC). Electron microscopic studies (EMS) on BCC and ELISAs for IgM, IgG and specific glycoprotein G-2 (gG-2) IgG were performed. HSV-2 DNA was detected by PCR on bladder tissue biopsies in 29.6% and 21.7% of group I and II respectively and it was also detected by PCR on BCC in 22.2% and 21.7% of group I and II respectively. EMS revealed HSV like particles in 16.6% of cases. IgG, specific gG-2 IgG and IgM were detected in 30%, 16% and 6% of cases respectively. The different assays were evaluated in relation to PCR on bladder tissue biopsies. The gG-2-based ELISA and EMS on BCC were found to be highly specific (97.3% and 100% respectively), with similar low sensitivity of approximately 54%. PCR on BCC was the most sensitive assay. The association of HSV-2 with bladder cancer is suggested especially in schistosomal patients.
本研究旨在调查单纯疱疹病毒 2 型（HSV-2）在埃及膀胱癌或膀胱炎患者中的流行情况，并评估不同的 HSV-2 诊断检测方法的性能。该研究纳入了 50 名患者：27 名膀胱癌患者（I 组）、23 名膀胱炎患者（II 组）和 20 名作为对照的受试者（III 组）。采用聚合酶链反应（PCR）检测膀胱组织和白细胞层细胞中的 HSV-2 DNA。对白细胞层细胞进行电子显微镜研究（EMS）和免疫球蛋白 IgM、IgG 和特异性糖蛋白 G-2（gG-2）IgG 的 ELISA 检测。PCR 检测膀胱组织活检标本中，I 组和 II 组分别有 29.6%和 21.7%的患者检测到 HSV-2 DNA，PCR 检测白细胞层细胞中，I 组和 II 组分别有 22.2%和 21.7%的患者检测到 HSV-2 DNA。EMS 在 16.6%的病例中发现类似疱疹的颗粒。分别有 30%、16%和 6%的患者检测到 IgG、特异性 gG-2 IgG 和 IgM。评估了不同的检测方法与膀胱组织活检 PCR 的相关性。基于 gG-2 的 ELISA 和白细胞层细胞的 EMS 检测具有很高的特异性（分别为 97.3%和 100%），敏感性相似，约为 54%。白细胞层细胞的 PCR 检测是最敏感的方法。HSV-2 与膀胱癌之间存在关联，特别是在埃及血吸虫病患者中。
