Paradžik Martina, Bučević-Popović Viljemka, Šitum Marijan, Jaing Crystal J, Degoricija Marina, McLoughlin Kevin S, Ismail Said I, Punda-Polić Volga, Terzić Janoš
Tumour Biol. 2014 Jan;35(1):567-72. doi: 10.1007/s13277-013-1079-2.
As the seventh most common human malignancy, bladder cancer represents a global health problem. In addition to well-recognized risk factors such as smoking and exposure to chemicals, various infectious agents have been implicated as cofactors in the pathogenesis of urothelial malignancies. The aim of the present study was to assess the possible association of viral infection and bladder cancer in Croatian patients. Biopsy specimens were collected from a total of 55 patients diagnosed with different stages of bladder cancer. Initial screening of DNA extracts for the presence of viruses on Lawrence Livermore Microbial Detection Array revealed Kaposi’s sarcoma-associated herpesvirus (KSHV) in each of three randomly chosen biopsy specimens. The prevalence of infection with KSHV among study population was then examined by KSHV-specific polymerase chain reaction (PCR) and immunoblotting. By nested PCR, KSHV DNA was detected in 55% of patients. KSHV, also known as human herpesvirus 8, is an infectious agent known to cause cancer. Its oncogenic potential is primarily recognized from its role in Kaposi’s sarcoma, but it has also been involved in pathogenesis of two lymphoproliferative disorders. A high prevalence of KSHV infection in our study indicates that KSHV may play a role in tumorigenesis of bladder cancer and warrants further studies.
膀胱癌作为人类第七大常见恶性肿瘤，是一个全球性的健康问题。除了吸烟和接触化学物质等公认的危险因素外，多种感染因子也被认为是尿路上皮恶性肿瘤发病机制中的辅助因素。本研究的目的是评估克罗地亚患者中病毒感染与膀胱癌之间可能存在的关联。从总共55例被诊断为不同阶段膀胱癌的患者中收集活检标本。在劳伦斯利弗莫尔微生物检测阵列上对DNA提取物进行病毒存在的初步筛查时，在随机选择的三个活检标本中均发现了卡波西肉瘤相关疱疹病毒（KSHV）。然后通过KSHV特异性聚合酶链反应（PCR）和免疫印迹法检测研究人群中KSHV感染的患病率。通过巢式PCR，在55%的患者中检测到KSHV DNA。KSHV也被称为人类疱疹病毒8型，是一种已知可致癌的感染因子。其致癌潜力主要从其在卡波西肉瘤中的作用中得到认可，但它也与两种淋巴增殖性疾病的发病机制有关。我们研究中KSHV感染的高患病率表明KSHV可能在膀胱癌的肿瘤发生中起作用，值得进一步研究。
