Clinic of Stomatology, Faculty of Medicine, Masaryk University Brno and St. Anne's University Hospital Brno, Brno, Czech Republic.
J Clin Periodontol. 2010 Feb;37(2):152-9. doi: 10.1111/j.1600-051X.2009.01523.x. Epub 2009 Dec 21.
Toll-like receptors (TLRs) belong to the pattern recognition receptors family of signal molecules that recognize conserved microbial structures. The aim of this study was to analyse polymorphisms in the TLR genes and their association with chronic periodontitis (CP).
Two polymorphisms (2408G/A, i.e. Arg753Gln and -16934A/T) in TLR-2 and three variants (-1486C/T, -1237C/T and+2848A/G) in the TLR-9 genes were studied in 222 patients with CP and 259 unrelated controls. All polymorphisms were detected using the polymerase chain reaction-restriction fragment length polymorphism methods. Subgingival bacterial colonization was investigated by the VariOr Dento test.
No significant differences were found in allele and genotype frequencies of all polymorphisms between patients and controls. Nevertheless, complex analysis revealed differences in TLR9 haplotype frequencies between both groups (p=0.001). Specifically, the haplotype T(-1486)/T(-1237)/A(2848) was significantly more frequent (9.6%versus 2.8%, p<0.000001) and the haplotype T(-1486)/T(-1237)/G (2848) of the TLR9 gene was less frequent (35.9%versus 43.3%, p=0.01) in patients than in controls. However, no significant relationships between periodontal pathogens, TLR polymorphisms and CP were found.
In conclusion, although no significant role of the TLR2 gene in periodontitis was found, our results indicate that TLR9 haplotypes may be associated with susceptibility to CP.
Toll 样受体（TLR）属于信号分子的模式识别受体家族，可识别保守的微生物结构。本研究旨在分析 TLR 基因的多态性及其与慢性牙周炎（CP）的关系。
在 222 例 CP 患者和 259 例无关对照中，研究了 TLR-2 基因中的两个多态性（2408G/A，即 Arg753Gln 和-16934A/T）和 TLR-9 基因中的三个变体（-1486C/T、-1237C/T 和+2848A/G）。所有多态性均采用聚合酶链反应-限制性片段长度多态性方法检测。通过 VariOr Dento 试验检测龈下细菌定植情况。
患者和对照组之间，所有多态性的等位基因和基因型频率均无显著差异。然而，复杂分析显示两组间 TLR9 单倍型频率存在差异（p=0.001）。具体而言，TLR9 基因的 T(-1486)/T(-1237)/A(2848) 单倍型明显更常见（9.6%比 2.8%，p<0.000001），而 T(-1486)/T(-1237)/G(2848) 单倍型则较少见（35.9%比 43.3%，p=0.01）。然而，牙周病原体、TLR 多态性与 CP 之间未发现显著相关性。
虽然 TLR2 基因在牙周炎中没有显著作用，但我们的结果表明 TLR9 单倍型可能与 CP 的易感性有关。
