Dipartimento PrIME and Centro Interdipartimentale Bioagromed, Università di Foggia, Foggia, Italy.
Zoonoses Public Health. 2010 Dec;57(7-8):e54-8. doi: 10.1111/j.1863-2378.2009.01304.x.
Molecular characterization of Giardia duodenalis cysts from humans and animals living in well-defined contexts is useful to study the circulation of isolates and represents a tool to evaluate zoonotic infection risk. The presence of giardiasis in children living in a disadvantaged and socially deprived small Rom community, as well in dogs roaming freely in the same context was carried out by microscopic analysis and beta-giardin gene amplification. Five out of 14 children were found positive at microscopic examination for G. duodenalis and six positive at PCR, while eight out of 14 dogs tested both microscopically and molecularly positive for G. duodenalis. Moreover, most of the children and dogs were symptomatic. Molecular characterization of Giardia positive samples from children and dogs showed 99.5% identity with Giardia Assemblage A1. The dog-specific genotypes C and D were not found. The findings of this survey provide the first European evidence to support the possible role of dogs in zoonotic transmission involving children and stray dogs in a closed context with very low standards of hygiene (i.e. Rom community), and these results show the need to monitor the health of marginal populations to safeguard ethnic minority groups.
对生活在明确背景下的人和动物的十二指肠贾第鞭毛虫包囊进行分子特征分析，有助于研究分离株的传播情况，是评估人畜共患感染风险的一种工具。在一个弱势和社会贫困的小型罗姆人社区生活的儿童以及在同一环境中自由游荡的狗中存在贾第虫病，通过显微镜分析和β-微管蛋白基因扩增进行了检测。14 名儿童中有 5 名在显微镜下检查为十二指肠贾第鞭毛虫阳性，6 名 PCR 阳性，14 名狗中有 8 名在显微镜和分子上均为十二指肠贾第鞭毛虫阳性。此外，大多数儿童和狗都有症状。对儿童和狗的贾第虫阳性样本进行的分子特征分析显示，与贾第虫 A1 聚集群的同一性为 99.5%。未发现狗特异性基因型 C 和 D。这项调查的结果提供了第一个欧洲证据，支持狗在涉及儿童和流浪狗的封闭环境中，通过非常低的卫生标准（即罗姆人社区）发生人畜共患传播的可能作用，这些结果表明需要监测边缘人群的健康状况，以保护少数民族群体。
