Department of Environmental Biotechnology, Water Technology Center, Karlsruhe, Germany.
Chemosphere. 2010 Jan;78(5):527-32. doi: 10.1016/j.chemosphere.2009.11.033. Epub 2009 Dec 29.
Cis-1,2-dichloroethene (cDCE) is a compound of concern at many chloroethene-contaminated sites, since it tends to accumulate during reductive dechlorination of the higher chlorinated ethenes. Stable carbon isotope fractionation during aerobic cDCE biodegradation was observed in groundwater microcosms under varying incubation conditions (room temperature/groundwater temperature; with/without inorganic nutrients), and resulted in an average stable carbon isotope enrichment factor of -15.2+/-0.5 per thousand. A new enrichment culture, obtained from groundwater microcosms, degraded cDCE concentrations up to 100mgL(-1), was active at temperatures between 4 and 23 degrees C, had a pH optimum of approximately 7, and could withstand prolonged periods (250d) of starvation. Microbial growth during degradation of cDCE as sole carbon and energy source was demonstrated by protein formation in mineral medium not containing any known auxiliary substrate. The obtained growth yield was 12.5+/-1.9g of proteinMol(-1) of cDCE, with a doubling time of 53+/-2h at 23 degrees C. Aerobic degradation of cDCE as sole carbon and energy source appears to be a promising biological process for site remediation.
顺-1,2-二氯乙烯（顺式-1,2-二氯乙烯，cDCE）是许多氯代烯烃污染场地中受到关注的化合物，因为它在较高氯代烯烃的还原脱氯过程中易于积累。在不同的培养条件下（室温/地下水温度；有/无无机养分），在地下水微宇宙中观察到好氧 cDCE 生物降解过程中的稳定碳同位素分馏，导致平均稳定碳同位素富集因子为-15.2±0.5 per thousand。从地下水微宇宙中获得的一种新的富集培养物，可降解高达 100mgL(-1)的 cDCE 浓度，在 4 至 23 摄氏度之间具有活性，最佳 pH 值约为 7，并且能够承受长时间（250d）的饥饿。通过在不含任何已知辅助底物的矿物培养基中形成蛋白质，证明了 cDCE 作为唯一碳源和能源时微生物的生长。在 23 摄氏度时，获得的生长产率为 12.5±1.9g 蛋白质 Mol(-1)的 cDCE，倍增时间为 53±2h。好氧降解 cDCE 作为唯一的碳源和能源，似乎是一种有前途的现场修复生物过程。
