Centro de Biotecnología-FEMSA, Tecnológico de Monterrey, Ave. Eugenio Garza Sada 2501 sur. Monterrey, N.L., C.P. 64849, Mexico.
Bioresour Technol. 2010 Apr;101(8):2837-44. doi: 10.1016/j.biortech.2009.10.047. Epub 2009 Dec 29.
This contribution examines the technical feasibility of producing high added value probiotic biomass from deproteinized and non-supplemented milk whey. The kinetics of growth of Lactobacillus casei in deproteinized goat milk whey was analyzed. Experiments in batch, continuous and fed-batch conditions were conducted in a 3 L fully instrumented bioreactor. Final substrate and biomass concentrations, yields and productivities are reported for different culture strategies. A kinetic analysis was conducted to characterize biomass production, product inhibition effects, and substrate consumption rates. Due to the strong product inhibition, fed-batch cultures at high biomass concentration rendered higher productivity (0.45 g L(-1) h(-1)) than batch and continuous cultures (0.11 g L(-1) h(-1)), complete lactose conversions (<1.0 g of lactose/L at the end of each fed-batch cycle), and a product with higher viable cell counts (2 x 10(10) cell/g of freeze-dried product). Based on our result, high-cell density fed-batch strategies are recommended for commercial production of probiotic L. casei biomass.
本研究旨在探讨从脱蛋白且未添加营养物的乳清中生产高附加值益生菌生物质的技术可行性。本文分析了干酪乳杆菌在脱蛋白山羊乳清中的生长动力学。在 3 L 全自动化生物反应器中，进行了分批、连续和补料分批培养实验。报告了不同培养策略下最终基质和生物质浓度、产率和生产率。进行了动力学分析以表征生物质生产、产物抑制效应和基质消耗速率。由于产物的强抑制作用，高生物质浓度的补料分批培养的生产率（0.45 g L(-1) h(-1)）高于分批和连续培养（0.11 g L(-1) h(-1)），完全转化乳糖（每批补料结束时乳糖浓度<1.0 g/L），且产物中活细胞计数更高（2 x 10(10) 细胞/g 冻干产物）。基于我们的研究结果，建议采用高细胞密度补料分批策略进行商业化生产益生菌干酪乳杆菌生物质。
