Nanjaiah Madhusudan, Rastogi Naveen Kumar, Devappa Somashekar
Microbiology and Fermentation Technology Department, CSIR-Central Food Technological and Research Institute, Mysore, Karnataka 570020 India.
Food Engineering Department, CSIR-Central Food Technological and Research Institute, Mysore, Karnataka 570020 India.
3 Biotech. 2024 Feb;14(2):49. doi: 10.1007/s13205-023-03899-z. Epub 2024 Jan 22.
In this study, NCIM 5752, a new isolate has been explored for probiotic properties and has shown significant bile salt hydrolase activity and cholesterol-reducing activity (56.7 ± 0.27%) in the presence of bile salts. It also tested negative for the production of lecithinase and gelatinase, indicating its non-pathogenic nature. The test strain was able to tolerate pH of 2.0 and 3.0 with 63.42 and 94.7% of the cells survived after 3 h. showed auto-aggregation of 85.3% and surface hydrophobicity of 22.5% in xylene and 19.4% in hexane. Paneer whey was explored as a basic raw material for alternative media formulation for growing lactic acid bacteria. Paneer whey was found to contain lactose (4.15%), protein (0.42%), and rich in mineral content. Response surface methodology was employed to optimize the medium composition with three independent variables yeast extract (X1), dextrose (X2), and dipotassium hydrogen phosphate (X3), and the response-Y was set to biomass obtained in terms of log CFU/ml. They were supplemented to paneer whey medium for growing this strain. The second-order polynomial regression model predicted that the maximum cell mass production of 11.30 ± 0.5 log CFU/ml at optimal composition of 16.22 g/L of yeast extract, 19.31 g/L of dextrose, and 2.12 g/L of dipotassium hydrogen phosphate in paneer whey medium. Experiments were conducted to validate the RSM results, and the biomass achieved was 11.27 ± 0.50 log CFU/ml, which is in close agreement with the yield predicted by the RSM. By applying the fermentation strategy, the biomass was increased to 5.56 ± 0.34 g/L dry cell weight corresponding to 11.58 ± 0.24 log CFU/ml. The newly optimized media was significantly cost-effective and produced 26.45% more biomass than the conventional MRS media. This optimized media may find application for the large-scale biomass production of probiotics.
The online version contains supplementary material available at 10.1007/s13205-023-03899-z.
在本研究中，对新分离株NCIM 5752的益生菌特性进行了探索，结果表明其在胆盐存在下具有显著的胆盐水解酶活性和降胆固醇活性（56.7±0.27%）。它还检测出卵磷脂酶和明胶酶呈阴性，表明其无致病性。测试菌株能够耐受pH值为2.0和3.0的环境，3小时后分别有63.42%和94.7%的细胞存活。在二甲苯和己烷中分别表现出85.3%的自聚集性、22.5%和19.4%的表面疏水性。对凝乳清作为乳酸菌替代培养基配方的基础原料进行了探索。发现凝乳清含有乳糖（4.15%）、蛋白质（0.42%），且矿物质含量丰富。采用响应面法对培养基组成进行优化，以酵母提取物（X1）、葡萄糖（X2）和磷酸氢二钾（X3）作为三个独立变量，将响应值-Y设定为以每毫升菌落形成单位（CFU/ml）对数表示的生物量。将它们添加到凝乳清培养基中以培养该菌株。二阶多项式回归模型预测，在凝乳清培养基中，当酵母提取物为16.22 g/L、葡萄糖为19.31 g/L、磷酸氢二钾为2.12 g/L的最佳组成时，最大细胞质量产量为11.30±0.5 log CFU/ml。进行实验以验证响应面法的结果，获得的生物量为11.27±0.50 log CFU/ml，与响应面法预测的产量非常接近。通过应用发酵策略，生物量增加到5.56±0.34 g/L干细胞重量，相当于11.58±0.24 log CFU/ml。新优化的培养基具有显著的成本效益，比传统的MRS培养基产生的生物量多26.45%。这种优化的培养基可能会用于益生菌的大规模生物量生产。
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