Animal Reproduction and Biotechnology Laboratory, Department of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Biol Reprod. 2010 Apr;82(4):725-35. doi: 10.1095/biolreprod.109.079467. Epub 2009 Dec 30.
Interferon tau (IFNT) from the ovine conceptus has paracrine actions on the endometrium that alter release of prostaglandin F(2alpha) (PGF) and protect the corpus luteum (CL). Antiviral activity in uterine vein blood and expression of interferon-stimulated genes (ISGs) in CL is greater in pregnant than in nonpregnant ewes. We hypothesized that IFNT contributes to antiviral activity in uterine vein blood and has endocrine actions on the CL. Preadsorption of IFNT with antiserum against recombinant ovine (ro) IFNT revealed that antiviral activity in uterine vein blood from pregnant ewes was mediated by IFNT. Endocrine actions of IFNT were examined after infusing either roIFNT or bovine serum albumin (BSA; 200 microg/24 h; mini-osmotic pump) into the uterine vein of nonpregnant ewes from Day 10 to Day 11 postestrus. The abundance of ISG15 mRNA and protein was greater in CL (P < 0.05) from ewes receiving 24-h roIFNT infusion compared to that from ewes receiving 24-h BSA infusion. Injection of PGF at 12 h following insertion of mini-osmotic pumps resulted in a decline in serum progesterone concentrations 6 through 12 h later in BSA-infused ewes; however, in roIFNT-infused ewes, a similar decline in progesterone concentrations at 6 h was followed by recovery to control values at 12 h. Ewes then received infusions (200 microg/day) of either roIFNT or BSA for 7 days beginning on Day 10 of the estrous cycle. All BSA-infused ewes returned to estrus by Day 19, whereas 80% of roIFNT-infused ewes maintained luteal-phase concentrations of progesterone through Day 32. In conclusion, IFNT is released from the uterus into the uterine vein and acts through an endocrine mechanism to induce ISGs in the CL and delay luteolysis.
绵羊胎盘中的干扰素 tau（IFNT）对子宫内膜具有旁分泌作用，改变前列腺素 F（2alpha）（PGF）的释放并保护黄体（CL）。与非妊娠母羊相比，妊娠母羊子宫静脉血液中的抗病毒活性和 CL 中干扰素刺激基因（ISGs）的表达更高。我们假设 IFNT有助于子宫静脉血液中的抗病毒活性，并对 CL 具有内分泌作用。用针对重组绵羊（ro）IFNT 的抗血清预先吸附 IFNT 表明，来自妊娠母羊的子宫静脉血液中的抗病毒活性是由 IFNT 介导的。在发情后第 10 天至第 11 天，将 roIFNT 或牛血清白蛋白（BSA；200μg/24h；迷你渗透泵）注入非妊娠母羊的子宫静脉中，研究 IFNT 的内分泌作用。与接受 24 小时 BSA 输注的母羊相比，接受 24 小时 roIFNT 输注的母羊 CL 中 ISG15mRNA 和蛋白质的丰度更高（P < 0.05）。在插入迷你渗透泵 12 小时后注射 PGF 会导致 BSA 输注母羊 6 至 12 小时后血清孕酮浓度下降；然而，在 roIFNT 输注母羊中，6 小时孕酮浓度的类似下降后恢复到 12 小时的对照值。然后，母羊在发情周期的第 10 天开始接受每天 200μg 的 roIFNT 或 BSA 输注 7 天。所有 BSA 输注的母羊在第 19 天恢复发情，而 80%的 roIFNT 输注的母羊在第 32 天维持黄体期孕酮浓度。总之，IFNT 从子宫释放到子宫静脉中，并通过内分泌机制作用于 CL 中的 ISGs 并延迟黄体溶解。
