评价长效孕激素注射对复发性奶牛干扰素相关基因表达的影响。
Evaluation of the effect of sustained-release progesterone injection on the expression of interferon-related genes in repeat-breeder dairy cows.
机构信息
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran.
出版信息
Vet Med Sci. 2024 Sep;10(5):e70005. doi: 10.1002/vms3.70005.
BACKGROUND
Repeat-breeder cows repeatedly fail to conceive after at least three attempts and return to oestrus at apparently normal intervals. Repeat-breeder cows cause economic losses in dairy farms in different ways.
OBJECTIVE
In the present study, we investigated the effect of sustained-release progesterone injection in two different doses on the expression of interferon-related genes in repeat-breeder dairy cows.
METHODS
A total of 96 repeat-breeder primiparous and multiparous cows were assigned among three groups: control group, inseminated and do not receive progesterone treatment; P and P groups, inseminated and received a single-intramuscular injection of 400 and 600 mg slow-release progesterone 5 days after insemination, respectively. Blood sampling was carried out on Day 20 after AI for progesterone measurement and evaluation of gene expression for ISG15, MX1 and MX2 genes.
RESULTS
One injection of sustained-release progesterone increased the expression of ISG15, MX1 and MX2 genes with differences between two different progesterone concentrations. For all three genes, the level of gene expression was higher in progesterone-supplemented group than in control group, when P and P groups considered together. The level of MX2 gene expression was significantly higher in pregnant cows than non-pregnant cows. There was a significant positive correlation between expression level of all three genes and blood progesterone concentration. The expression level of ISG15 gene showed a significant positive correlation with MX1 and MX2 gene expression.
CONCLUSION
The use of this sustained-release progesterone is simple and can be used in repeat-breeder cows to improve fertility.
背景
重复配种奶牛在至少三次配种后仍未能受孕，并在明显正常的间隔时间内再次发情。重复配种奶牛以不同的方式给奶牛场造成经济损失。
目的
本研究旨在探讨两种不同剂量的缓释孕酮注射对重复配种奶牛干扰素相关基因表达的影响。
方法
将 96 头初产和经产的重复配种奶牛随机分为三组：对照组，配种后不接受孕酮处理；P 和 P 组，配种后 5 天分别单次肌肉注射 400 和 600mg 缓释孕酮。在人工授精后第 20 天采血，检测孕酮水平，并评估 ISG15、MX1 和 MX2 基因的表达。
结果
单次注射缓释孕酮可增加 ISG15、MX1 和 MX2 基因的表达，且两种不同孕酮浓度之间存在差异。对于所有三个基因，与对照组相比，添加孕酮组的基因表达水平更高，当将 P 和 P 组一起考虑时。在怀孕奶牛中，MX2 基因的表达水平明显高于非怀孕奶牛。所有三个基因的表达水平与血液孕酮浓度呈显著正相关。ISG15 基因的表达水平与 MX1 和 MX2 基因的表达水平呈显著正相关。
结论
使用这种缓释孕酮简单且可用于重复配种奶牛以提高生育能力。
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