Department of Histology and Embryology, Sun Yat-sen University, Guangzhou, Guangdong, PR China.
Oncol Rep. 2010 Feb;23(2):387-95.
To investigate the anti-pituitary adenoma effect of secalonic acid D (SAD) extracting from marine microorganisms, we used MTT colorimetric method to evaluate the proliferation of GH3 cells treated by SAD, the time- and dose-dependent effects and the value of IC50 were recorded. Hoechst staining, Tunel and flow cytometry methods were used to analyze the apoptosis rate of GH3 cells treated by SAD. Western blotting, RT-PCR and caspase inhibitor (Z-VAD-FMK) were used to investigate the possible mechanism of SAD induced apoptosis and the expression of growth hormone (GH). The results showed that SAD has a time- and dose-dependent effect on GH3 cells and the cytotoxic effect was mainly through apoptosis. The mechanisms were partly through the activity of caspase family and also G1/S phase block. In addition, SAD also can suppress the expression levels of growth hormone in GH3 cells, however, the RT-PCR results showed that the mechanism was not through changing the expression of GH mRNA. We concluded that SAD may be a potential anti-pituitary tumor drug and further in vivo studies should be performed.
为了研究从海洋微生物中提取的 secalonic 酸 D（SAD）对垂体腺瘤的抑制作用，我们采用 MTT 比色法检测 SAD 对 GH3 细胞增殖的影响，记录 SAD 的时间和剂量依赖性作用及其 IC50 值。Hoechst 染色、Tunel 和流式细胞术方法分析 SAD 处理的 GH3 细胞的凋亡率。Western blot、RT-PCR 和 caspase 抑制剂（Z-VAD-FMK）用于研究 SAD 诱导凋亡和生长激素（GH）表达的可能机制。结果表明，SAD 对 GH3 细胞具有时间和剂量依赖性作用，其细胞毒性作用主要通过凋亡实现。部分机制与 caspase 家族的活性有关，也与 G1/S 期阻滞有关。此外，SAD 还可以抑制 GH3 细胞中生长激素的表达水平，但 RT-PCR 结果表明，其机制并非通过改变 GH mRNA 的表达。我们得出结论，SAD 可能是一种有潜力的抗垂体肿瘤药物，应进一步进行体内研究。
