Lee Sun Haeng, Kwak Sung Chul, Kim Dong Kwan, Park Sang Woug, Kim Hyun Soo, Kim Young-Sik, Lee Donghun, Lee Ju Won, Lee Chang Gon, Lee Hae Kyung, Cho Sung-Min, Shin Yu Jeong, Lee Jin Yong, Kim Hocheol, Chang Gyu Tae
Department of Clinical Korean Medicine, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea; Department of Pediatrics of Korean Medicine, Kyung Hee University Medical Center, Seoul 02447, Republic of Korea.
Korea Institute of Science and Technology for Eastern Medicine (KISTEM), NeuMed Inc., Seoul 02440, Republic of Korea.
Evid Based Complement Alternat Med. 2016;2016:9389028. doi: 10.1155/2016/9389028. Epub 2016 Jan 26.
The present study was to evaluate the effects of Huang Bai, Zhi Mu, Mai Ya, and Xia Ku Cao on hormone using the GT1-7 and GH3 cells. The GT1-7 and GH3 cell lines were incubated with DW; DMSO; and 30, 100, or 300 μg/mL of one of the four extract solutions in serum-free media for 24 hours. The MTT assay was performed to determine the cytotoxicity of the four herbs. The GT1-7 and GH3 cells were incubated in DW, estradiol (GT1-7 only), or noncytotoxic herb solutions in serum-free medium for 24 hours. A quantitative RT-PCR and western blot were performed to measure the GnRH expression in GT1-7 cells and GH expression in GH3 cells. Huang Bai, Zhi Mu, Xia Ku Cao, and Mai Ya inhibited the GnRH mRNA expression in GT1-7 cells, whereas Huang Bai enhanced GH mRNA expression in GH3 cells. Additionally, Xia Ku Cao inhibited GnRH protein expression in GT1-7 cells and Huang Bai promoted GH protein expression in GH3 cells. The findings suggest that Huang Bai can delay puberty by inhibiting GnRH synthesis in the hypothalamus while also accelerating growth by promoting GH synthesis and secretion in the pituitary.
本研究旨在利用GT1-7和GH3细胞评估黄柏、知母、麦芽和夏枯草对激素的影响。将GT1-7和GH3细胞系在无血清培养基中与蒸馏水、二甲基亚砜以及四种提取物溶液之一的30、100或300μg/mL孵育24小时。进行MTT试验以确定这四种草药的细胞毒性。将GT1-7和GH3细胞在无血清培养基中的蒸馏水、雌二醇（仅针对GT1-7）或无细胞毒性的草药溶液中孵育24小时。进行定量逆转录聚合酶链反应和蛋白质印迹法以测量GT1-7细胞中促性腺激素释放激素（GnRH）的表达以及GH3细胞中生长激素（GH）的表达。黄柏、知母、夏枯草和麦芽抑制GT1-7细胞中GnRH mRNA的表达，而黄柏增强GH3细胞中GH mRNA的表达。此外，夏枯草抑制GT1-7细胞中GnRH蛋白的表达，黄柏促进GH3细胞中GH蛋白的表达。这些发现表明，黄柏可通过抑制下丘脑GnRH的合成来延迟青春期，同时还可通过促进垂体中GH的合成和分泌来加速生长。
