Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China.
J Zhejiang Univ Sci B. 2010 Jan;11(1):61-70. doi: 10.1631/jzus.B0900174.
Strawberry anthracnose, caused by Colletotrichum spp., is a major disease of cultivated strawberry. This study identifies 31 isolates of Colletotrichum spp. which cause strawberry anthracnose in Zhejiang Province and Shanghai City, China. Eleven isolates were identified as C. acutatum, 10 as C. gloeosporioides and 10 as C. fragariae based on morphological characteristics, phylogenetic and sequence analyses. Species-specific polymerase chain reaction (PCR) and enzyme digestion further confirmed the identification of the Colletotrichum spp., demonstrating that these three species are currently the causal agents of strawberry anthracnose in the studied regions. Based on analysis of rDNA internal transcribed spacers (ITS) sequences, sequences of all C. acutatum were identical, and little genetic variability was observed between C. fragariae and C. gloeosporioides. However, the conservative nature of the MvnI specific site from isolates of C. gloeosporioides was confirmed, and this site could be used to differentiate C. gloeosporioides from C. fragariae.
草莓炭疽病由胶孢炭疽菌引起，是一种严重影响草莓种植的病害。本研究鉴定了来自中国浙江和上海的 31 株胶孢炭疽菌分离株，导致草莓炭疽病。根据形态特征、系统发育和序列分析，11 株被鉴定为尖孢炭疽菌，10 株为胶孢炭疽菌，10 株为草莓炭疽菌。种特异性聚合酶链反应（PCR）和酶切进一步证实了炭疽菌的鉴定，表明这三个种是目前研究区域草莓炭疽病的病原菌。基于 rDNA 内转录间隔区（ITS）序列分析，所有尖孢炭疽菌的序列完全相同，而草莓炭疽菌和胶孢炭疽菌之间观察到的遗传变异很小。然而，胶孢炭疽菌菌株中的 MvnI 特异性位点的保守性得到了确认，并且该位点可用于区分胶孢炭疽菌和草莓炭疽菌。
