College of Agronomy & Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, P.R. China.
Southeast Chongqing Academy of Agricultural Sciences, Fuling 408000, P.R. China.
Plant Dis. 2021 Oct;105(10):3025-3036. doi: 10.1094/PDIS-10-20-2114-RE. Epub 2021 Nov 23.
Strawberry anthracnose, caused by species, is a major fungal disease threatening the strawberry industry in Sichuan Province of southwestern China. However, research on identification of species associated with strawberry anthracnose in Sichuan remains scarce. In this study, 73 representative strains were isolated from diseased leaves, stolons, petioles, and crowns of 11 major strawberry-planting localities in Sichuan Province. Based on morphological characteristics and multiloci phylogenetic analysis, the strains were identified as three distinct species: (53 strains, 72.60%), (17 strains, 23.29%), and (3 strains, 4.11%). Among them, was the most ubiquitous and dominant species, whereas was restricted to Chongzhou. Importantly, our pathogenicity tests showed that and can infect both leaves and stolons, whereas was only pathogenic to leaves. Interestingly, although the sexual stage of was not observed in this study, it still exhibited the strongest virulence to strawberry compared with and . This is the first study to characterize species causing strawberry anthracnose and evaluate their pathogenicity in Sichuan Province of southwestern China, which will provide a better strategy for accurate diagnosis and management of anthracnose disease in strawberry.
草莓炭疽病由炭疽病菌引起，是威胁中国西南地区四川省草莓产业的主要真菌病害。然而，关于四川草莓炭疽病相关炭疽病菌的鉴定研究仍然较少。本研究从四川省 11 个主要草莓种植区的病叶、匍匐茎、叶柄和冠部共分离到 73 株代表菌株。基于形态特征和多位点系统发育分析，这些菌株被鉴定为三个不同的种：胶孢炭疽菌（53 株，72.60%）、草莓炭疽菌（17 株，23.29%）和围小丛壳菌（3 株，4.11%）。其中，胶孢炭疽菌最为普遍和优势，而草莓炭疽菌仅限于崇州。重要的是，我们的致病性试验表明，胶孢炭疽菌和草莓炭疽菌均可侵染叶片和匍匐茎，而围小丛壳菌仅对叶片致病。有趣的是，尽管本研究未观察到胶孢炭疽菌的有性阶段，但它仍然表现出比草莓炭疽菌和围小丛壳菌更强的对草莓的致病力。这是首次对引起草莓炭疽病的炭疽病菌种进行特征描述，并评估其在四川省的致病性，这将为草莓炭疽病的准确诊断和管理提供更好的策略。
