Ricketts D, Turnbull L, Ryall G, Bakhshi R, Rawson N S, Gazet J C, Nolan C, Coombes R C
Medical Oncology Department, St. George's Hospital Medical School, London, United Kingdom.
Cancer Res. 1991 Apr 1;51(7):1817-22.
We have studied estrogen receptor (ER) and progesterone receptor (PR) in normal breast by immunocytochemistry using tissue biopsies and fine needle aspirates (FNA) and, in the case of ER, by enzyme immunoassay. For ER we found a high degree of reproducibility for biopsies taken from the upper outer quadrant: FNA, r = 0.56 (P less than 0.002); tissue section immunocytochemistry, r = 0.89 (P less than 0.0001); and enzyme immunoassay, r = 0.76 (P less than 0.0001). For PR, FNA (r = 0.56, P less than 0.002) and tissue section (r = 0.97, P less than 0.0001) were also found to be reproducible techniques. Using enzyme immunoassay, we were able to measure ER accurately in normal breast tissue. In 59 samples we found a range of 0-37 fmol/mg cytosol protein (mean, 4 fmol/mg). In an age-matched group of 126 women with breast cancer, we found a significantly higher ER [range, 0-139 fmol/mg; mean, 37 fmol/mg (P less than 0.001)]. We then analyzed the ER and PR content of FNAs obtained from the upper outer quadrant of the normal breast in 143 normal women. We found that in only 23 of 143 samples (16%) were greater than or equal to 50% epithelial cells stained. There was a relationship between ER and PR (P = 0.03) and a higher ER content in European women than in non-European women (P less than 0.03). The PR content was related to high body mass index (P less than 0.02) and family history of breast cancer (P = 0.04). Samples tended to be more frequently ER positive by FNA if taken in the follicular phase of the menstrual cycle. We conclude that, although the levels of ER and PR are low in normal breast, they can be accurately measured. There is significant variation of ER and PR with several clinical parameters.
我们通过免疫细胞化学方法，利用组织活检和细针穿刺抽吸物（FNA）对正常乳腺中的雌激素受体（ER）和孕激素受体（PR）进行了研究，对于ER，还采用了酶免疫测定法。对于ER，我们发现从乳腺外上象限获取的活检样本具有高度的可重复性：FNA检测，r = 0.56（P < 0.002）；组织切片免疫细胞化学检测，r = 0.89（P < 0.0001）；酶免疫测定法，r = 0.76（P < 0.0001）。对于PR，FNA（r = 0.56，P < 0.002）和组织切片（r = 0.97，P < 0.0001）也被发现是可重复的技术。使用酶免疫测定法，我们能够准确测量正常乳腺组织中的ER。在59个样本中，我们发现ER水平范围为0 - 37 fmol/mg胞浆蛋白（平均值为4 fmol/mg）。在126名年龄匹配的乳腺癌女性患者中，我们发现ER水平显著更高[范围为0 - 139 fmol/mg；平均值为37 fmol/mg（P < 0.001）]。然后，我们分析了143名正常女性正常乳腺外上象限FNA中的ER和PR含量。我们发现，在143个样本中，只有23个（16%）样本中≥50%的上皮细胞呈染色阳性。ER和PR之间存在相关性（P = 0.03），欧洲女性的ER含量高于非欧洲女性（P < 0.03）。PR含量与高体重指数（P < 0.02）和乳腺癌家族史（P = 0.04）相关。如果在月经周期的卵泡期采集样本，FNA检测样本的ER阳性率往往更高。我们得出结论，尽管正常乳腺中ER和PR的水平较低，但它们可以被准确测量。ER和PR在多个临床参数方面存在显著差异。
