Chen S C, Chou C K, Wong F H, Chang C M, Hu C P
Graduate Institute of Microbiology and Immunology, National Yang-Ming Medical College, Taipei, Taiwan, Republic of China.
Cancer Res. 1991 Apr 1;51(7):1898-903.
The growth-stimulatory effects of epidermal growth factor (EGF), transforming growth factor alpha (TGF-alpha), and insulin-like growth factor-I (IGF-I) on the human esophageal carcinoma cell line CE48T/VGH were evaluated. Under serum-free conditions, EGF, TGF-alpha, and IGF-I promoted 3.6- to 4.1-fold increased cell proliferation. Scatchard analyses and Northern blot hybridization revealed that both the EGF/TGF-alpha receptor and the IGF-I receptor were overexpressed in CE48T/VGH cells. Furthermore, ligand-dependent autophosphorylation of the EGF receptor and the IGF-I receptor was clearly detected using antireceptor and antiphosphotyrosine antibodies. Autocrine regulation was strongly indicated by the following evidence: (a) CE48T/VGH cells were found to express TGF-alpha and IGF-I genes, (b) serum-free conditioned medium promoted the growth of CE48T/VGH cells and stimulated the autophosphorylation of the EGF/TGF-alpha receptor and the IGF-I receptor, and (c) the addition of IGF-I receptor antibodies significantly suppressed CE48T/VGH cell growth under serum-free conditions. Our studies suggest that the overexpression of EGF and IGF-I receptors and autocrine growth regulation may concertedly control the proliferation of esophageal carcinoma cells.
评估了表皮生长因子（EGF）、转化生长因子α（TGF-α）和胰岛素样生长因子-I（IGF-I）对人食管癌细胞系CE48T/VGH的生长刺激作用。在无血清条件下，EGF、TGF-α和IGF-I可使细胞增殖增加3.6至4.1倍。Scatchard分析和Northern印迹杂交显示，CE48T/VGH细胞中EGF/TGF-α受体和IGF-I受体均过度表达。此外，使用抗受体和抗磷酸酪氨酸抗体可清楚地检测到EGF受体和IGF-I受体的配体依赖性自磷酸化。以下证据强烈表明存在自分泌调节：（a）发现CE48T/VGH细胞表达TGF-α和IGF-I基因，（b）无血清条件培养基可促进CE48T/VGH细胞生长并刺激EGF/TGF-α受体和IGF-I受体的自磷酸化，（c）添加IGF-I受体抗体可在无血清条件下显著抑制CE48T/VGH细胞生长。我们的研究表明，EGF和IGF-I受体的过度表达以及自分泌生长调节可能共同控制食管癌细胞的增殖。
