Cleary Orlaith B, Trumble Troy N, Merritt Kelly A, Brown Murray P
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA.
Am J Vet Res. 2010 Jan;71(1):33-40. doi: 10.2460/ajvr.71.1.33.
To investigate the effects of exercise and osteochondral injury on concentrations of carboxy-terminal telopeptide fragments of type II collagen (CTX-II) in synovial fluid (SF) and serum of Thoroughbred racehorses and to compare findings with radiographic and arthroscopic scores of joint injury severity.
78 Thoroughbreds with (n = 38) and without (40) osteochondral injury.
Serum and metacarpophalangeal or carpal joint SF samples were collected from noninjured horses before and at the end of 5 to 6 months of race training (pre- and postexercise samples, respectively) and from horses with osteochondral injury (1 joint assessed/horse). Synovial fluid and serum CTX-II concentrations were determined by use of an ELISA. Radiographic and arthroscopic scores of joint injury severity were determined for the injured horses.
The CTX-II concentrations in SF and SF:serum CTX-II ratio were significantly higher for horses with joint injuries, compared with pre- and postexercise findings in noninjured horses. Serum CTX-II concentrations in postexercise and injured-horse samples were significantly lower than values in pre-exercise samples. On the basis of serum and SF CTX-II concentrations and SF:serum CTX-II ratio, 64% to 93% of serum and SF samples were correctly classified into their appropriate group (pre-exercise, postexercise, or injured-joint samples). In horses with joint injuries, arthroscopic scores were positively correlated with radiographic scores, but neither score correlated with SF or serum CTX-II concentration.
Results suggested that serum and SF CTX-II concentrations and SF:serum CTX-II ratio may be used to detect cartilage degradation in horses with joint injury.
研究运动和骨软骨损伤对纯种赛马滑液（SF）和血清中II型胶原羧基末端肽片段（CTX-II）浓度的影响，并将结果与关节损伤严重程度的X线和关节镜评分进行比较。
78匹纯种马，其中38匹有骨软骨损伤，40匹无骨软骨损伤。
从未受伤的马匹在5至6个月的比赛训练前和结束时采集血清和掌指关节或腕关节滑液样本（分别为运动前和运动后样本），并从有骨软骨损伤的马匹（每匹马评估1个关节）采集样本。使用酶联免疫吸附测定法测定滑液和血清CTX-II浓度。对受伤马匹进行关节损伤严重程度的X线和关节镜评分。
与未受伤马匹的运动前和运动后结果相比，关节损伤马匹的滑液中CTX-II浓度和滑液：血清CTX-II比值显著更高。运动后样本和受伤马匹样本中的血清CTX-II浓度显著低于运动前样本中的值。根据血清和滑液CTX-II浓度以及滑液：血清CTX-II比值，64%至93%的血清和滑液样本被正确分类到相应组（运动前样本组、运动后样本组或受伤关节样本组）。在有关节损伤的马匹中，关节镜评分与X线评分呈正相关，但两种评分均与滑液或血清CTX-II浓度无关。
结果表明，血清和滑液CTX-II浓度以及滑液：血清CTX-II比值可用于检测关节损伤马匹的软骨降解情况。
