Vencovský J
Výzkumný ústav chorob revmatických, Praha.
Cas Lek Cesk. 1991 Jan 25;130(4):108-11.
Rheumatoid factors (RF) detected by agglutination methods (latex fixation test, haemagglutination test) were found in a group of patients with rheumatoid arthritis which started in advanced age (RAVV) less frequently than in a group with RA in middle age (RAMV). The author describes the ELISA method for assessment of RF which makes it possible to estimate immunoglobulin classes RF (IgM-RF, IgA-RF, IgG-RF). By this method RF were detected in class IgM in 15% of patients and in some immunoglobulin classes in 20 per cent where are RF negative when assessed by agglutination methods. The ELISA method detects probably also so-called "hidden" RF. In the group of healthy subjects above 60 years at least one class of RF rises to 17.5%. On correlation with the clinical status an association was found with the degree of affection according to the functional score, with the presence of rheumatoid nodes and muscular affection in RA. Patients with RA treated with Methotrexate and Sulfasalazine tend to have declining IgG RF levels.
通过凝集法（乳胶凝集试验、血凝试验）检测类风湿因子（RF）时，发现一组老年起病的类风湿关节炎患者（RAVV）中RF的检出率低于中年类风湿关节炎患者组（RAMV）。作者描述了一种用于评估RF的ELISA方法，该方法能够估计RF的免疫球蛋白类别（IgM-RF、IgA-RF、IgG-RF）。通过这种方法，在15%的患者中检测到IgM类RF，在20%通过凝集法评估为RF阴性的患者中检测到某些免疫球蛋白类别的RF。ELISA方法可能还能检测到所谓的“隐匿性”RF。在60岁以上的健康受试者组中，至少一类RF升高至17.5%。与临床状态相关分析发现，根据功能评分，其与病情严重程度、类风湿结节的存在以及类风湿关节炎中的肌肉病变有关。接受甲氨蝶呤和柳氮磺胺吡啶治疗的类风湿关节炎患者的IgG RF水平往往会下降。
