Zlabinger G J, Bröll H
Institut für Immunologie der Universität Wien.
Z Rheumatol. 1988 Mar-Apr;47(2):107-12.
Sera from patients with definite or classic rheumatoid arthritis (RA) were tested for their reactivity to the Fc fragment of human IgG using a solid phase Elisa with specificity for rheumatoid factors (RF) of classes IgM, IgG and IgA. For all RF classes tested the Elisa was reproducible both within and between different assays. IgM-RF-serum concentrations correlated well to the Waaler Rose titers (r = 0.88, p less than 0.001). No difference in RF-serum concentrations of any Ig class could be detected between patients with seronegative erosive RA and controls. In the sera of seropositive RA patients, however, significantly higher concentrations of all RF-classes were observed (IgM RF: seropos. x- = 454 +/- 805 IU/ml, seroneg. x- = 1.3 +/- 0.6 IU/ml, p less than 0.0005; IgG RF: seropos. x- = 17 +/- 28 U/ml, seroneg. x- = 2 +/- 1.8 U/ml, p less than 0.005; IgA RF: seropos. x- = 300 +/- 577 U/ml, seroneg. x- = 4 +/- 3.6 U/ml, p less than 0.005). In our seronegative RA patients at least one antigen of the HLA-B5 CREG could be observed. Together with the well documented frequency of HLA-DR4 in patients with seropositive RA, this finding points to possible, genetically determined regulative mechanisms, which control the presence of autoantibodies within rheumatoid arthritis.
采用对IgM、IgG和IgA类类风湿因子（RF）具有特异性的固相酶联免疫吸附测定（ELISA）法，检测确诊或典型类风湿关节炎（RA）患者血清对人IgG Fc片段的反应性。对于所有检测的RF类别，ELISA法在不同检测内部和之间均可重复。IgM-RF血清浓度与瓦勒-罗斯滴度相关性良好（r = 0.88，p < 0.001）。在血清阴性侵蚀性RA患者与对照组之间，未检测到任何Ig类别的RF血清浓度有差异。然而，在血清阳性RA患者的血清中，观察到所有RF类别的浓度均显著更高（IgM RF：血清阳性x̅ = 454 ± 805 IU/ml，血清阴性x̅ = 1.3 ± 0.6 IU/ml，p < 0.0005；IgG RF：血清阳性x̅ = 17 ± 28 U/ml，血清阴性x̅ = 2 ± 1.8 U/ml，p < 0.005；IgA RF：血清阳性x̅ = 300 ± 577 U/ml，血清阴性x̅ = 4 ± 3.6 U/ml，p < 0.005）。在我们的血清阴性RA患者中，可观察到至少一种HLA-B5 CREG抗原。连同血清阳性RA患者中HLA-DR4的高发生率记录，这一发现指向可能的、由基因决定的调节机制，该机制控制类风湿关节炎中自身抗体的存在。
