Division of Bioengineering, Faculty of Engineering, National University of Singapore, Singapore, Singapore.
Nanomedicine. 2010 Jun;6(3):486-95. doi: 10.1016/j.nano.2009.11.004. Epub 2010 Jan 4.
The photodynamic effect of upconversion nanoparticles loaded with a photosensitizer was studied on murine bladder cancer cells (MB49). Mesoporous silica was coated onto sodium yttrium fluoride upconversion nanocrystals to form a core-shell structure and then loaded with the photosensitizer zinc (II)-phthalocyanine into the porous silica. The nanoparticles displayed a uniform spherical shape with an average diameter of about 50 nm and showed good dispersibility in water. Intracellular uptake study in MB49 cells revealed a time- and concentration-dependent accumulation of these nanoparticles. Upon irradiation with 980-nm near-infrared light, their efficiency in activating the loaded zinc (II)-phthalocyanine to generate singlet oxygen molecules was confirmed in live cells. The cytotoxic effect of the released singlet oxygen from the nanoplatform was proven by cell viability assay, confocal microscopy, DNA agarose gel electrophoresis, cytochrome c-releasing assay, and prostate-specific antigen-enzyme-linked immunosorbent assay, all of which showed a strong photodynamic effect of the nanoparticles on MB49 cells. This suggests the efficacy of sodium yttrium fluoride upconversion nanoparticles as a carrier for photosensitizers and their use in photodynamic therapy of cancer and some other diseases.
In this study, the photodynamic effect of upconversion nanoparticles loaded with a photosensitizer was investigated on murine bladder cancer cells, with strongly positive results, which may pave its way to future clinical use in malignant tumors and potentially other diseases.
研究了负载光敏剂的上转换纳米粒子对小鼠膀胱癌细胞（MB49）的光动力效应。介孔硅包覆在钇钠氟上转换纳米晶上，形成核壳结构，然后将光敏剂锌（II）酞菁载入多孔硅中。纳米粒子呈均匀的球形，平均直径约为 50nm，在水中具有良好的分散性。在 MB49 细胞中的细胞内摄取研究表明，这些纳米粒子的积累具有时间和浓度依赖性。用 980nm 近红外光照射后，在活细胞中证实了它们激活负载的锌（II）酞菁产生单线态氧分子的效率。从纳米平台释放的单线态氧的细胞毒性效应通过细胞活力测定、共聚焦显微镜、DNA 琼脂糖凝胶电泳、细胞色素 c 释放测定和前列腺特异性抗原酶联免疫吸附测定得到证实，所有这些都表明纳米粒子对 MB49 细胞具有很强的光动力效应。这表明了上转换纳米粒子作为光敏剂载体的功效，以及它们在癌症和其他一些疾病的光动力治疗中的应用。
在这项研究中，研究了负载光敏剂的上转换纳米粒子对小鼠膀胱癌细胞的光动力效应，结果非常积极，这可能为其未来在恶性肿瘤和潜在其他疾病中的临床应用铺平道路。
