急性心肌梗死患者血清中锰超氧化物歧化酶的浓度

Concentration of Mn-superoxide dismutase in serum in acute myocardial infarction.

作者信息

Usui A, Kato K, Tsuboi H, Sone T, Sassa H, Abe T

机构信息

Department of Thoracic Surgery, Nagoya University School of Medicine, Japan.

出版信息

Clin Chem. 1991 Mar;37(3):458-61.

PMID:2004457

Abstract

Serum concentrations of manganese-containing superoxide dismutase (Mn-SOD; EC 1.15.1.1), a mitochondrial enzyme present in high concentrations in the heart, were measured successively by enzyme immunoassay in 18 patients with acute myocardial infarction (AMI) and in eight with angina pectoris. Results were compared with creatine kinase (EC 2.7.3.2) isoenzyme MB (CK-MB) concentrations in the same specimens. The mean (and SD) serum Mn-SOD concentration in 120 healthy adults was 77.5 (18) micrograms/L. Mn-SOD concentrations exceeding 150 micrograms/L (greater than mean + 4 SD) were considered above-normal. In patients with AMI, the Mn-SOD concentration was 80 (16.8) micrograms/L on admission and increased gradually after the first hospital day. The peak, 260 (109) microgram/L, occurred on the fourth hospital day, at which time the Mn-SOD concentrations in all AMI patients were above normal. Thereafter the Mn-SOD concentration decreased slowly, but still was above normal in 14 of 18 patients on the seventh hospital day. On the other hand, in patients with angina pectoris, the Mn-SOD concentration was 78 (11) micrograms/L on admission and did not increase significantly [peak value 97.5 (42) micrograms/L on the fourth hospital day]. The serum concentration of Mn-SOD is a potentially useful marker for estimating cardiac mitochondrial damage and for diagnosing AMI, especially in the late phase.

摘要

含锰超氧化物歧化酶（Mn-SOD；EC 1.15.1.1）是一种在线粒体中高浓度存在的酶，在心脏中含量丰富。我们采用酶免疫分析法，对18例急性心肌梗死（AMI）患者和8例心绞痛患者的血清Mn-SOD浓度进行了连续测定。并将结果与同一标本中的肌酸激酶（EC 2.7.3.2）同工酶MB（CK-MB）浓度进行了比较。120名健康成年人的血清Mn-SOD平均（及标准差）浓度为77.5（18）μg/L。Mn-SOD浓度超过150μg/L（大于均值 + 4个标准差）被视为高于正常水平。AMI患者入院时Mn-SOD浓度为80（16.8）μg/L，入院后第一天开始逐渐升高。峰值为260（109）μg/L，出现在入院后第四天，此时所有AMI患者的Mn-SOD浓度均高于正常水平。此后，Mn-SOD浓度缓慢下降，但在入院后第七天，18例患者中有14例仍高于正常水平。另一方面，心绞痛患者入院时Mn-SOD浓度为78（11）μg/L，且未显著升高[入院后第四天峰值为97.5（42）μg/L]。血清Mn-SOD浓度是评估心脏线粒体损伤和诊断AMI的一个潜在有用标志物，尤其是在晚期。