Usui A, Kato K, Murase M, Maeda M, Koyama T, Tanaka M, Takeuchi E, Abe T
Department of Thoracic Surgery, Nagoya University School of Medicine, Japan.
Jpn Circ J. 1992 Dec;56(12):1206-13. doi: 10.1253/jcj.56.1206.
Concentrations of Manganese-containing superoxide dismutase (Mn-SOD) were measured perioperatively by enzyme immunoassay in serial samples of arterial and coronary sinus blood and urine taken from 18 patients undergoing mitral valve surgery. The mean Mn-SOD concentration in the arterial blood samples was 66.2 (SD 16.1 ng/ml) at induction of anesthesia, increased gradually after reperfusion and peaked on the 2nd post-operative day [150 (SD 58.3) ng/ml]. The mean concentration of Mn-SOD in the coronary sinus blood samples was significantly higher than in the arterial samples only at the 6th hour after reperfusion [97 (SD 21.8) ng/ml vs 90.3 (SD 20.9) ng/ml, p < 0.05]. Although concentrations of Mn-SOD in blood did not increase in 8 patients who underwent midline sternotomy for a mediastinal tumor, they increased dramatically in 3 patients who sustained a perioperative myocardial infarction. During open heart surgery the peak values of plasma Mn-SOD concentrations were correlated to that of plasma creatine kinase-MB concentrations (r = 0.5532, n = 18, p < 05) and cardiac ischemic period (r = 0.5186, n = 18, p < 05). Although the meaning of an increase in plasma Mn-SOD concentrations during open heart surgery is not clarified, it may be released from the heart and anywhere also in the body damaged during cardiopulmonary bypass.
采用酶免疫分析法对18例接受二尖瓣手术患者围手术期动脉血、冠状窦血及尿液系列样本中的含锰超氧化物歧化酶（Mn-SOD）浓度进行了测定。麻醉诱导时动脉血样本中Mn-SOD的平均浓度为66.2（标准差16.1 ng/ml），再灌注后逐渐升高，并在术后第2天达到峰值[150（标准差58.3）ng/ml]。仅在再灌注后第6小时，冠状窦血样本中Mn-SOD的平均浓度显著高于动脉样本[97（标准差21.8）ng/ml对90.3（标准差20.9）ng/ml，p<0.05]。虽然8例因纵隔肿瘤行胸骨正中切开术的患者血液中Mn-SOD浓度未升高，但3例围手术期发生心肌梗死的患者其浓度显著升高。在心脏直视手术期间，血浆Mn-SOD浓度的峰值与血浆肌酸激酶-MB浓度（r=0.5532，n=18，p<0.05）及心脏缺血时间（r=0.5186，n=18，p<0.05）相关。虽然心脏直视手术期间血浆Mn-SOD浓度升高的意义尚不清楚，但它可能是从心脏以及体外循环期间身体任何受损部位释放出来的。
