Dianita Roza, Jantan Ibrahim, Amran Athirah Z, Jalil Juriyati
Drug and Herbal Research Center, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia.
Molecules. 2015 Mar 16;20(3):4746-63. doi: 10.3390/molecules20034746.
The study was designed to evaluate the cardioprotective effects of the standardized aqueous and 80% ethanol extracts of Labisia pumila var. alata (LPva) in isoproterenol (ISO)-induced myocardial infarction (MI) in rats. The extracts were administered to Wistar rats orally for 28 days with three doses (100, 200 and 400 mg/kg of body weight) prior to ISO (85 mg/kg)-induced MI in two doses on day 29 and 30. The sera and hearts were collected for biochemical and histopathological analysis after the rats were sacrificed 48 h after the first induction. The main components of the extracts, gallic acid, alkylresorcinols and flavonoids were identified and quantitatively analyzed in the extracts by using a validated reversed phase HPLC method. The extracts showed significant protective effects as pretreated rats showed a significant dose-dependent decrease (p < 0.05) in cardiac enzyme activities, i.e., cardiac troponin I (cTnI), creatine kinase MB isoenzyme (CK-MB), lactate dehydrogenase (LDH), alanine transaminase (ALT) and aspartate transaminase (AST), when compared with ISO-control rats. There were significant rises (p < 0.05) in the activity of oxidase enzymes, i.e., glutathione peroxide (GPx), catalase (CAT) and superoxide dismutase (SOD) of the pretreated rats, when compared with ISO-control group. Histopathological examination showed an improvement in membrane cell integrity in pre-treated rats compared to untreated rats. The major components of LPva extracts can be used as their biomarkers and contributed to the cardioprotective effects against ISO-induced MI rats.
本研究旨在评估标准水提取物和80%乙醇提取物对异丙肾上腺素（ISO）诱导的大鼠心肌梗死（MI）的心脏保护作用。在第29天和第30天，以两种剂量（85mg/kg）的ISO诱导大鼠心肌梗死之前，将提取物以三种剂量（100、200和400mg/kg体重）口服给予Wistar大鼠28天。在首次诱导后48小时处死大鼠后，收集血清和心脏进行生化和组织病理学分析。采用经过验证的反相高效液相色谱法对提取物中的主要成分没食子酸、烷基间苯二酚和黄酮类化合物进行了鉴定和定量分析。提取物显示出显著的保护作用，因为与ISO对照组大鼠相比，预处理大鼠的心脏酶活性，即心肌肌钙蛋白I（cTnI）、肌酸激酶MB同工酶（CK-MB）、乳酸脱氢酶（LDH）、丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）显著呈剂量依赖性降低（p<0.05）。与ISO对照组相比，预处理大鼠的氧化酶活性，即谷胱甘肽过氧化物酶（GPx）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）显著升高（p<0.05）。组织病理学检查显示，与未处理大鼠相比，预处理大鼠的膜细胞完整性有所改善。小叶榕提取物的主要成分可作为其生物标志物，并有助于对ISO诱导的MI大鼠产生心脏保护作用。
