Zimmerman T S, Arroyove C M, Müller-Eberhard H J
J Exp Med. 1971 Dec 1;134(6):1591-600. doi: 10.1084/jem.134.6.1591.
Evidence for the involvement of the sixth component of complement (C6) in normal blood coagulation is provided by the description of a coagulation abnormality in rabbits with a genetic C6 deficiency and by its correction with highly purified preparations of C6. Whole blood clotting time in glass or plastic was prolonged and prothrombin consumption was decreased in blood from the deficient animals. Other parameters of blood coagulation were normal, including prothrombin time, partial thromboplastin time, specific clotting factor activities, platelet factor III function, platelet count, and bleeding time. Clotting time and prothrombin consumption became normal when physiologic amounts of highly purified C6 were added to the deficient blood. Partial consumption of C6 hemolytic activity, with a time course similar to the consumption of prothrombin, was demonstrated during the clotting of normal human blood.
遗传性C6缺乏的兔子出现凝血异常，并通过用高度纯化的C6制剂纠正该异常，从而证明补体第六成分（C6）参与正常血液凝固。缺乏C6的动物血液在玻璃或塑料中全血凝固时间延长，凝血酶原消耗减少。血液凝固的其他参数正常，包括凝血酶原时间、部分凝血活酶时间、特定凝血因子活性、血小板因子III功能、血小板计数和出血时间。当向缺乏C6的血液中添加生理量的高度纯化C6时，凝固时间和凝血酶原消耗恢复正常。在正常人血液凝固过程中，C6溶血活性出现部分消耗，其时间进程与凝血酶原的消耗相似。
