菌血症导致实验性肺炎球菌性脑膜炎中海马细胞凋亡。
Bacteremia causes hippocampal apoptosis in experimental pneumococcal meningitis.
机构信息
Department of Clinical Microbiology, Copenhagen University Hospital Herlev, Herlev, Denmark.
出版信息
BMC Infect Dis. 2010 Jan 3;10:1. doi: 10.1186/1471-2334-10-1.
BACKGROUND
Bacteremia and systemic complications both play important roles in brain pathophysiological alterations and the outcome of pneumococcal meningitis. Their individual contributions to the development of brain damage, however, still remain to be defined.
METHODS
Using an adult rat pneumococcal meningitis model, the impact of bacteremia accompanying meningitis on the development of hippocampal injury was studied. The study comprised of the three groups: I. Meningitis (n = 11), II. meningitis with attenuated bacteremia resulting from iv injection of serotype-specific pneumococcal antibodies (n = 14), and III. uninfected controls (n = 6).
RESULTS
Pneumococcal meningitis resulted in a significantly higher apoptosis score 0.22 (0.18-0.35) compared to uninfected controls (0.02 (0.00-0.02), Mann Whitney test, P = 0.0003). Also, meningitis with an attenuation of bacteremia by antibody treatment resulted in significantly reduced apoptosis (0.08 (0.02-0.20), P = 0.01) as compared to meningitis.
CONCLUSIONS
Our results demonstrate that bacteremia accompanying meningitis plays an important role in the development of hippocampal injury in pneumococcal meningitis.
背景
菌血症和全身并发症在肺炎球菌性脑膜炎的脑病理生理改变和结局中均发挥重要作用。然而，它们对脑损伤发展的各自贡献仍有待明确。
方法
采用成年大鼠肺炎球菌性脑膜炎模型，研究伴随脑膜炎的菌血症对海马损伤发展的影响。研究包括三组：I. 脑膜炎（n = 11），II. 脑膜炎伴因 iv 注射血清型特异性肺炎球菌抗体导致的菌血症减弱（n = 14），和 III. 未感染对照（n = 6）。
结果
与未感染对照（0.02（0.00-0.02），Mann-Whitney 检验，P = 0.0003）相比，肺炎球菌性脑膜炎导致明显更高的凋亡评分（0.22（0.18-0.35）。而且，通过抗体治疗减弱菌血症的脑膜炎导致的凋亡明显减少（0.08（0.02-0.20），P = 0.01），与脑膜炎相比。
结论
我们的结果表明，伴随脑膜炎的菌血症在肺炎球菌性脑膜炎中海马损伤的发展中发挥重要作用。
Zotero 插件