Reddy Venky Sreedhar, Rao D K Venkata, Rajasekharan Ram
Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India.
Biochim Biophys Acta. 2010 Apr;1801(4):455-61. doi: 10.1016/j.bbalip.2009.12.005. Epub 2010 Jan 4.
Lysophosphatidic acid (LPA) acts as a signaling molecule that regulates diverse cellular processes and it can rapidly be metabolized by phosphatase and acyltransferase. LPA phosphatase gene has not been identified and characterized in plants so far. The BLAST search revealed that the At3g03520 is similar to phospholipase family, and distantly related to bacterial phosphatases. The conserved motif, (J)4XXXNXSFD, was identified in both At3g03520 like phospholipases and acid phosphatases. In silico expression analysis of At3g03520 revealed a high expression during phosphate starvation and abiotic stresses. This gene was overexpressed in Escherichia coli and shown to posses LPA specific phosphatase activity. These results suggest that this gene possibly plays a role in signal transduction and storage lipid synthesis.
溶血磷脂酸（LPA）作为一种信号分子，可调节多种细胞过程，并且它能被磷酸酶和酰基转移酶快速代谢。到目前为止，LPA磷酸酶基因尚未在植物中被鉴定和表征。BLAST搜索显示，At3g03520与磷脂酶家族相似，且与细菌磷酸酶有较远的亲缘关系。在At3g03520类磷脂酶和酸性磷酸酶中都鉴定出了保守基序（J）4XXXNXSFD。对At3g03520的电子表达分析显示，在磷饥饿和非生物胁迫期间其表达量较高。该基因在大肠杆菌中过表达，并显示具有LPA特异性磷酸酶活性。这些结果表明，该基因可能在信号转导和储存脂质合成中发挥作用。
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