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拟南芥非特异性磷脂酶C1：特性及其在热应激反应中的作用

Arabidopsis non-specific phospholipase C1: characterization and its involvement in response to heat stress.

作者信息

Krčková Zuzana, Brouzdová Jitka, Daněk Michal, Kocourková Daniela, Rainteau Dominique, Ruelland Eric, Valentová Olga, Pejchar Přemysl, Martinec Jan

机构信息

Institute of Experimental Botany, The Czech Academy of Sciences Prague, Czech Republic ; Department of Biochemistry and Microbiology, University of Chemistry and Technology, Prague Prague, Czech Republic.

Institute of Experimental Botany, The Czech Academy of Sciences Prague, Czech Republic.

出版信息

Front Plant Sci. 2015 Nov 4;6:928. doi: 10.3389/fpls.2015.00928. eCollection 2015.

DOI:10.3389/fpls.2015.00928
PMID:26581502
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4631941/
Abstract

The Arabidopsis non-specific phospholipase C (NPC) protein family is encoded by the genes NPC1 - NPC6. It has been shown that NPC4 and NPC5 possess phospholipase C activity; NPC3 has lysophosphatidic acid phosphatase activity. NPC3, 4 and 5 play roles in the responses to hormones and abiotic stresses. NPC1, 2 and 6 has not been studied functionally yet. We found that Arabidopsis NPC1 expressed in Escherichia coli possesses phospholipase C activity in vitro. This protein was able to hydrolyse phosphatidylcholine to diacylglycerol. NPC1-green fluorescent protein was localized to secretory pathway compartments in Arabidopsis roots. In the knock out T-DNA insertion line NPC1 (npc1) basal thermotolerance was impaired compared with wild-type (WT); npc1 exhibited significant decreases in survival rate and chlorophyll content at the seventh day after heat stress (HS). Conversely, plants overexpressing NPC1 (NPC1-OE) were more resistant to HS compared with WT. These findings suggest that NPC1 is involved in the plant response to heat.

摘要

拟南芥非特异性磷脂酶C（NPC）蛋白家族由NPC1 - NPC6基因编码。研究表明，NPC4和NPC5具有磷脂酶C活性；NPC3具有溶血磷脂酸磷酸酶活性。NPC3、4和5在植物对激素和非生物胁迫的响应中发挥作用。目前尚未对NPC1、2和6进行功能研究。我们发现，在大肠杆菌中表达的拟南芥NPC1在体外具有磷脂酶C活性。该蛋白能够将磷脂酰胆碱水解为二酰基甘油。NPC1-绿色荧光蛋白定位于拟南芥根中的分泌途径区室。与野生型（WT）相比，敲除T-DNA插入系NPC1（npc1）的基础耐热性受损；热胁迫（HS）后第7天，npc1的存活率和叶绿素含量显著降低。相反，与WT相比，过表达NPC1的植株（NPC1-OE）对HS更具抗性。这些发现表明，NPC1参与了植物对热的响应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c98d/4631941/74aa6be928cb/fpls-06-00928-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c98d/4631941/5017eda58077/fpls-06-00928-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c98d/4631941/5dc629776046/fpls-06-00928-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c98d/4631941/bbbb00c7a0cd/fpls-06-00928-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c98d/4631941/28fe8b2769bc/fpls-06-00928-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c98d/4631941/50c2b54facbb/fpls-06-00928-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c98d/4631941/4e03ef8169bd/fpls-06-00928-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c98d/4631941/74aa6be928cb/fpls-06-00928-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c98d/4631941/5017eda58077/fpls-06-00928-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c98d/4631941/5dc629776046/fpls-06-00928-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c98d/4631941/bbbb00c7a0cd/fpls-06-00928-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c98d/4631941/28fe8b2769bc/fpls-06-00928-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c98d/4631941/50c2b54facbb/fpls-06-00928-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c98d/4631941/4e03ef8169bd/fpls-06-00928-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c98d/4631941/74aa6be928cb/fpls-06-00928-g007.jpg

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