Department of Biology, Laboratory of Biology and Biotechnology of Microorganisms, Microbiology and Environmental Toxicology Unit, Faculty of Sciences Semlalia Marrakech, University Cadi Ayyad, PO Box 2390, Marrakech 40000, Morocco.
Ecotoxicol Environ Saf. 2010 Jul;73(5):762-70. doi: 10.1016/j.ecoenv.2009.12.015. Epub 2009 Dec 31.
In this study, a 12-day growth trial was conducted to compare the effect of the variation in microcystin (MC) composition in two Microcystis aeruginosa bloom samples on the growth performance and MC accumulation/transfer in the common carp (Cyprinus carpio L.) larvae. Fish were fed Artemia salina nauplii that had been preexposed to extracts from two M. aeruginosa natural blooms with different microcystins (MCs) profiles. Bloom A had MC-LR as major toxin (74.05%) while bloom B had a diversity of MC (MC-RR; MC-(H4)YR; MC-YR; MC-LR; MC-FR; MC-WR) with no dominance of MC-LR. Newly-hatched Artemia nauplii were exposed separately to the two M. aeruginosa extracts A and B (100 microg L(-1)EqMC-LR) for 2h. The MC concentration in the nauplii was 73.60+/-7.88ngEqMC-LRg(-1)FW (n=4, mean+/-SE) for bloom A and 87.04+/-10.31ngEqMC-LRg(-1)FW for bloom B. These contaminated nauplii were given at the same ration to different groups (A and B) of fish larvae. Larval weight and length from day 9 were significantly different between groups A and B, and in both cases lower than that of a control group fed non-exposed nauplii. MCs accumulation by larvae, inversely correlated with the growth performance, was also significantly different between groups A and B (37.43+/-2.61 and 54.55+/-3.01ngEqMC-LRg(-1) FW, respectively, at the end of the experimental period). These results indicate that MC profile of a bloom may have differential effects on toxin accumulation/transfer and toxicity.
在这项研究中，进行了为期 12 天的生长试验，以比较两种铜绿微囊藻水华样本中微囊藻毒素（MC）组成的变化对鲤鱼（Cyprinus carpio L.）幼鱼生长性能和 MC 积累/转移的影响。研究人员用已预先暴露于两种具有不同微囊藻毒素（MC）图谱的铜绿微囊藻天然水华提取物的卤虫无节幼体来喂养鱼。水华 A 中 MC-LR 是主要毒素（74.05%），而水华 B 中则含有多种 MC（MC-RR；MC-(H4)YR；MC-YR；MC-LR；MC-FR；MC-WR），没有 MC-LR 的主导地位。新孵化的卤虫无节幼体分别暴露于两种铜绿微囊藻提取物 A 和 B（100 微克 L（-1）EqMC-LR）2 小时。水华 A 中卤虫无节幼体的 MC 浓度为 73.60+/-7.88ngEqMC-LRg（-1）FW（n=4，平均值+/-SE），水华 B 中则为 87.04+/-10.31ngEqMC-LRg（-1）FW。这些受污染的卤虫无节幼体以相同的比例被喂给不同组（A 和 B）的鱼幼体。A 组和 B 组的幼鱼体重和体长在第 9 天有显著差异，且均低于对照组（喂食未暴露的卤虫无节幼体）。鱼幼体对 MC 的积累与生长性能呈反比，A 组和 B 组之间也存在显著差异（实验期末，A 组和 B 组分别为 37.43+/-2.61 和 54.55+/-3.01ngEqMC-LRg（-1）FW）。这些结果表明，水华的 MC 图谱可能对毒素积累/转移和毒性产生不同的影响。
