London Metropolitan University, Institute for Health Research and Policy, 166-220 Holloway Road, London N7 8DB, UK.
Int J Antimicrob Agents. 2010 Mar;35(3):255-60. doi: 10.1016/j.ijantimicag.2009.11.017. Epub 2009 Dec 31.
In the last two decades, antimicrobial peptides (AMPs) have been gaining attention as antimicrobial alternatives to chemical food preservatives and commonly used antibiotics. Lactobacillus acidophilus n.v. Er 317/402 strain Narine produces a small AMP with a molecular weight of 1.1kDa, designated acidocin LCHV. In this study, the AMP was extremely heat stable (90min at 130 degrees C), was active over a wide pH range and was found to be sensitive to proteolytic enzymes (trypsin, pepsin and proteinase K). Acidocin LCHV has a broad spectrum of activity both against Gram-positive and Gram-negative pathogens, including several that are classified as Especially Dangerous Infections by the World Health Organization as well as meticillin-resistant Staphylococcus aureus (MRSA) and Clostridium difficile. Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS) was used to determine the molecular mass and sequence of the purified peptide. Complete killing with immediate impact on cells was observed within a very short period of time (10min).
在过去的二十年中，抗菌肽（AMPs）作为化学食品防腐剂和常用抗生素的替代品，受到了越来越多的关注。嗜酸乳杆菌 n.v. Er 317/402 菌株 Narine 产生一种分子量为 1.1kDa 的小 AMP，命名为嗜酸菌素 LCHV。在这项研究中，该 AMP 具有极强的热稳定性（在 130°C 下 90 分钟），在很宽的 pH 范围内都具有活性，并且对蛋白酶（胰蛋白酶、胃蛋白酶和蛋白酶 K）敏感。嗜酸菌素 LCHV 对革兰氏阳性和革兰氏阴性病原体具有广谱活性，包括世界卫生组织列为特别危险感染的几种病原体，以及耐甲氧西林金黄色葡萄球菌（MRSA）和艰难梭菌。基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF/MS）用于确定纯化肽的分子量和序列。在很短的时间内（10 分钟）即可观察到对细胞的立即完全杀伤作用。
