Bogovic-Matijasić B, Rogelj I, Nes I F, Holo H
Biotechnical Faculty, Zootechnical Department, Domzale, Slovenia.
Appl Microbiol Biotechnol. 1998 May;49(5):606-12. doi: 10.1007/s002530051221.
Lactobacillus acidophilus LF221 produced bacteriocin-like activity against different bacteria including some pathogenic and food-spoilage species. Besides some lactic acid bacteria, the following species were inhibited: Bacillus cereus, Clostridium sp., Listeria innocua, Staphylococcus aureus, Streptococcus D. L. acidophilus LF221 produced at least two bacteriocins, acidocin LF221 A and acidocin LF221 B, which were purified by ammonium sulphate precipitation, ion-exchange chromatography, hydrophobic interaction and reverse-phase FPLC. The antibacterial substances were heat-stable, sensitive to proteolytic enzymes (trypsin, pepsin, pronase, proteinase K) and migrated as 3500- to 5000-Da proteins on sodium dodecyl sulphate/polyacrylamide gel electrophoresis. The sequences of 46 amino-terminal amino acid residues of peptide A and 35 of peptide B were determined. Among the residues identified, no modified amino acids were found. No significant homology was found between the amino acid sequences of acidocin LF221 A and other bacteriocins of lactic acid bacteria and 26% homology was found between acidocin LF221 B and brevicin 27. L. acidophilus LF221 may be of interest as a probiotic strain because of its human origin and inhibition of pathogenic bacteria, especially clostridium difficile.
嗜酸乳杆菌LF221对包括一些致病和食品腐败菌在内的不同细菌产生类细菌素活性。除了一些乳酸菌外，还抑制了以下菌种：蜡样芽孢杆菌、梭菌属、无害李斯特菌、金黄色葡萄球菌、D群链球菌。嗜酸乳杆菌LF221产生了至少两种细菌素，即嗜酸菌素LF221 A和嗜酸菌素LF221 B，它们通过硫酸铵沉淀、离子交换色谱、疏水相互作用和反相快速蛋白质液相色谱法进行纯化。这些抗菌物质对热稳定，对蛋白水解酶（胰蛋白酶、胃蛋白酶、链霉蛋白酶、蛋白酶K）敏感，在十二烷基硫酸钠/聚丙烯酰胺凝胶电泳上以3500至5000道尔顿的蛋白质形式迁移。测定了肽A的46个氨基末端氨基酸残基和肽B的35个氨基酸残基的序列。在鉴定出的残基中，未发现修饰氨基酸。嗜酸菌素LF221 A的氨基酸序列与其他乳酸菌细菌素之间未发现显著同源性，嗜酸菌素LF221 B与短乳杆菌素27之间有26%的同源性。嗜酸乳杆菌LF221因其源自人类且能抑制病原菌，尤其是艰难梭菌，可能作为一种益生菌菌株受到关注。
