Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, G. Bonchev 9, Sofia 1113, Bulgaria.
Peptides. 2011 Jul;32(7):1477-83. doi: 10.1016/j.peptides.2011.05.001. Epub 2011 Jun 22.
Hemolymph of Rapana venosa snails is a complex mixture of biochemically and pharmacologically active components such as peptides and proteins. Antimicrobial peptides are gaining attention as antimicrobial alternatives to chemical food preservatives and commonly used antibiotics. Therefore, for the first time we have explored the isolation, identification and characterisation of 11 novel antimicrobial peptides produced by the hemolymph of molluscs. The isolated peptides from the hemolymph applying ultrafiltration and reverse-phase high-performance liquid chromatography (RP-HPLC) have molecular weights between 3000 and 9500 Da, determined by mass spectrometric analysis. The N-terminal sequences of the peptides identified by Edman degradation matched no peptides in the MASCOT search database, indicating novel proline-rich peptides. UV spectra revealed that these substances possessed the characteristics of protein peptides with acidic isoelectric points. However, no Cotton effects were observed between 190 and 280 nm by circular dichroism spectroscopy. Four of the pro-rich peptides also showed strong antimicrobial activities against tested microorganisms including Gram-positive and Gram-negative bacteria.
文蛤血淋巴是一种生化和药理活性成分（如肽和蛋白质）的复杂混合物。抗菌肽作为化学食品防腐剂和常用抗生素的替代品，正受到越来越多的关注。因此，我们首次探索了从软体动物血淋巴中分离、鉴定和表征 11 种新型抗菌肽。通过超滤和反相高效液相色谱（RP-HPLC）从血淋巴中分离出的肽的分子量在 3000 至 9500 Da 之间，这是通过质谱分析确定的。通过 Edman 降解鉴定的肽的 N-末端序列与 MASCOT 搜索数据库中的任何肽都不匹配，表明这是新型富含脯氨酸的肽。紫外光谱表明，这些物质具有酸性等电点的蛋白质肽的特征。然而，圆二色性光谱在 190 至 280nm 之间未观察到任何 Cotton 效应。四种富含脯氨酸的肽对测试的微生物（包括革兰氏阳性菌和革兰氏阴性菌）也具有很强的抗菌活性。
