Okinawa Institute of Science and Technology (OIST), Suzaki 12-2, Uruma, Okinawa 904-2234, Japan.
Anal Biochem. 2010 Apr 15;399(2):305-7. doi: 10.1016/j.ab.2009.12.036. Epub 2010 Jan 4.
A high recovery microsampling probe based on microdialysis was devised. The new probe showed a high recovery (100%) of peptides in vitro at different perfusion flow rates (0.1-1.0 microl/min). At a high flow rate, 1.0 microl/min, a 10-fold increased in recovery of peptides compared to the conventional microdialysis probe was achieved. A probe made of a low molecular weight cutoff membrane is suitable for filtering off proteins. The new probe can be a useful tool for high recovery of peptides from living tissues.
设计了一种基于微透析的高回收率微采样探针。新探针在不同灌注流速（0.1-1.0μl/min）下显示出对肽的高回收率（100%）。在高流速下，与传统微透析探针相比，肽的回收率提高了 10 倍。由低分子量截止膜制成的探针适用于过滤蛋白质。这种新的探针可以成为从活组织中高回收率提取肽的有用工具。
