Division of Gastrointestinal Pathophysiology, Department of Bioscience, Institute of Natural Medicine, University of Toyama, Toyama, Japan.
Biol Pharm Bull. 2010;33(1):142-5. doi: 10.1248/bpb.33.142.
Mucosal inflammation in ulcerative colitis (UC) is presumed to be regulated primarily by type 2 T helper cell immune responses and mucosal mast cells in the colon are thought to play an important role in the pathogenesis of the mucosal inflammation. Saireito, a Japanese herbal medicine of standardized quality, originating from traditional Chinese medicine (Kampo medicine), is composed of two different Kampo medicines (shosaikoto and goreisan) and is often used for UC in Japan. In this study, we examined the direct effects of these Kampo medicines and their constituents on the antigen-induced degranulation of mucosal-type mast cells. Mucosal-type murine bone marrow-derived mast cells (mBMMCs) were pretreated by these drugs for 24 h, and immunoglobulin E (IgE) receptor-triggered degranulation of mBMMCs was assessed by beta-hexosaminidase release. Goreisan showed inhibitory effects on degranulation of mBMMCs in a dose-dependent manner. Among the five constituent medicinal herbs of goreisan, Poria and Polyporus had the inhibitory effects on mBMMCs. Ergosterol, a principal and common component of Poria and Polyporus, also suppressed the degranulation of mBMMCs. Our results provide a molecular basis to explain a portion of the beneficial therapeutic properties of saireito on UC.
溃疡性结肠炎（UC）中的黏膜炎症被认为主要是由 2 型辅助性 T 细胞免疫反应调节的，而结肠黏膜肥大细胞被认为在黏膜炎症的发病机制中起重要作用。薬味方剂清肠汤是一种源自中药（汉方药）的标准化质量的日本草药，由两种不同的汉方药（小柴胡汤和六君子汤）组成，在日本常用于治疗 UC。在这项研究中，我们研究了这些汉方药及其成分对黏膜型肥大细胞抗原诱导脱颗粒的直接作用。用这些药物预处理黏膜型鼠骨髓来源的肥大细胞（mBMMC）24 小时，通过β-己糖胺酶释放评估 IgE 受体触发的 mBMMC 脱颗粒。六君子汤以剂量依赖的方式显示出对 mBMMC 脱颗粒的抑制作用。在六君子汤的五种组成草药中，茯苓和猪苓对 mBMMC 具有抑制作用。茯苓和猪苓的主要和常见成分麦角甾醇也抑制 mBMMC 的脱颗粒。我们的研究结果为解释清肠汤对 UC 的部分有益治疗特性提供了分子基础。
