Kim Su-Jin, Shin Hyun-Ji, Lee Geun-Hyuk, Kim Dae-Seung, Kim Hye-Lin, Park Jinbong, Jung Yunu, Youn Dong-Hyun, Kang Jongwook, Hong Seung-Heon, Um Jae-Young
Department of Cosmeceutical Science, Daegu Hanny University, Yugok‑dong, Kyungsan 712‑715, Republic of Korea.
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Iksan, Jeonbuk 570‑749, Republic of Korea.
Mol Med Rep. 2015 Sep;12(3):3549-3556. doi: 10.3892/mmr.2015.3824. Epub 2015 May 22.
Ulcerative colitis (UC) is a type of inflammatory bowel disease and is considered a chronic gastrointestinal disorder. Igongsan (IGS) is a Korean herbal medicine, which has been used to treat digestive disorders. However, the ameliorative effect and molecular mechanisms of IGS in intestinal inflammation have not yet been studied in detail. The present study aimed to investigate the protective effects of IGS and its constituent, ergosterol, in a mouse model of dextran sulfate sodium (DSS)‑induced colitis. Colitis was induced in mice by supplementing their drinking water with 5% (w/v) DSS for 7 days. The effects of IGS were then determined on DSS‑induced clinical signs of colitis, including weight loss, colon shortening, diarrhea and obscure/gross bleeding. In addition, the effects of IGS were determined on the expression levels of inflammation‑associated genes in the colon tissue of DSS‑treated mice. The results of the present study demonstrated that mice treated with DSS exhibited marked clinical symptoms, including weight loss and reduced colon length. Treatment with IGS attenuated these symptoms and also suppressed the expression levels of tumor necrosis factor‑α and interleukin‑6, as well as the expression of cyclooxygenase‑2 in the colon tissue of DSS‑treated mice. IGS also reduced the activation of the transcription factor nuclear factor‑κB p65 in the colon tissue of DSS‑treated mice. In addition, ergosterol was shown to attenuate the DSS‑induced clinical symptoms of colitis in mice. In conclusion, the present study provided experimental evidence that IGS may be a useful therapeutic drug for patients with UC.
溃疡性结肠炎（UC）是一种炎症性肠病，被认为是一种慢性胃肠疾病。益母散（IGS）是一种韩方草药，已被用于治疗消化系统疾病。然而，IGS在肠道炎症中的改善作用及其分子机制尚未得到详细研究。本研究旨在探讨IGS及其成分麦角固醇在葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎模型中的保护作用。通过在小鼠饮用水中补充5%（w/v）DSS 7天来诱导结肠炎。然后确定IGS对DSS诱导的结肠炎临床症状的影响，包括体重减轻、结肠缩短、腹泻和隐血/肉眼出血。此外，还确定了IGS对DSS处理小鼠结肠组织中炎症相关基因表达水平的影响。本研究结果表明，用DSS处理的小鼠表现出明显的临床症状，包括体重减轻和结肠长度缩短。用IGS治疗可减轻这些症状，并抑制DSS处理小鼠结肠组织中肿瘤坏死因子-α和白细胞介素-6的表达水平，以及环氧合酶-2的表达。IGS还降低了DSS处理小鼠结肠组织中转录因子核因子-κB p65的激活。此外，麦角固醇被证明可减轻DSS诱导的小鼠结肠炎临床症状。总之，本研究提供了实验证据，表明IGS可能是UC患者的一种有用治疗药物。
