Noguchi-Yachide Tomomi, Dodo Kosuke, Aoyama Hiroshi, Fujimoto Haruka, Hori Masatoshi, Hashimoto Yuichi, Kobayashi Hisayoshi
Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, Japan.
Chem Pharm Bull (Tokyo). 2010 Jan;58(1):129-34. doi: 10.1248/cpb.58.129.
Fusarielin A (FSA), isolated from culture of Fusarium sp. K432, has anti-fungal/anti-angiogenic activity. Based on our previous structure-activity relationship studies, we prepared FSA-immobilized affinity gel. The results of affinity chromatography with this gel indicated that tubulin and actin are FSA-binding proteins. The binding affinities of tubulin and actin for FSA were estimated by the use of surface plasmon resonance spectroscopy.
从镰刀菌属K432菌株培养物中分离得到的镰刀菌素A（FSA）具有抗真菌/抗血管生成活性。基于我们之前的构效关系研究，我们制备了固定有FSA的亲和凝胶。用这种凝胶进行亲和层析的结果表明，微管蛋白和肌动蛋白是FSA结合蛋白。通过表面等离子体共振光谱法估算了微管蛋白和肌动蛋白对FSA的结合亲和力。
