Kariwa Hiroaki, Tkachenko Evgeniy A, Morozov Vyacheslav G, Seto Takahiro, Tanikawa Yoichi, Kolominov Sergey I, Belov Sergey N, Nakamura Ichiro, Hashimoto Nobuo, Balakiev Alexander E, Dzagurnova Tamara K, Daud Nur Hardy Bin Abu, Miyashita Daisuke, Medvedkina Olga A, Nakauchi Mina, Ishizuka Mariko, Yoshii Kentaro, Yoshimatsu Kumiko, Arikawa Jiro, Takashima Ikuo
Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
J Vet Med Sci. 2009 Dec;71(12):1569-78. doi: 10.1292/jvms.001569.
European Russia is a highly endemic area of hemorrhagic fever with renal syndrome (HFRS), a rodent-borne zoonotic disease, caused by hantaviruses. In total, 145 small mammals of four species (Myodes glareolus, Apodemus flavicollis, A. agrarius, and A. uralensis) were trapped in the Samara region of European Russia in August 2005 and examined for the presence of hantavirus (HV). Anti-HV antibodies were found in six of 68 (8.8%) M. glareolus and in one of 19 (5.3%) A. flavicollis by indirect immunofluorescent antibody assay (IFA). The Puumala virus (PUUV), which is one of the hantavirus species, was detected in the lungs of seven M. glareolus by RT-PCR. The virus S-segment was extremely similar (96.2% to 99.3%) to the sequence found in a fatal case of HFRS in the Samara region. Phylogenetic analyses of S and M segments showed that the Samara PUUVs form a cluster within the Russian Volga lineage and apparently differ from other European PUUVs. Anti-PUUV antibodies were found in blood sera from seven HFRS patients and from one undiagnosed patient from the Samara region, using IFA and an enzyme-linked immunosorbent assay (ELISA). These data suggest that the bank vole M. glareolus is a primary natural reservoir and vector for PUUV, which is the main causative agent of HFRS in humans in the Samara region.
俄罗斯欧洲部分是肾综合征出血热（HFRS）的高流行区，这是一种由汉坦病毒引起的鼠传人畜共患病。2005年8月，在俄罗斯欧洲部分的萨马拉地区共捕获了4种（黄颈姬鼠、棕背䶄、黑线姬鼠和乌拉尔姬鼠）145只小型哺乳动物，并检测是否存在汉坦病毒（HV）。通过间接免疫荧光抗体试验（IFA），在68只黄颈姬鼠中的6只（8.8%）和19只棕背䶄中的1只（5.3%）中发现了抗HV抗体。通过逆转录聚合酶链反应（RT-PCR）在7只黄颈姬鼠的肺中检测到了汉坦病毒种类之一的普马拉病毒（PUUV）。该病毒的S片段与在萨马拉地区一例HFRS死亡病例中发现的序列极为相似（96.2%至99.3%）。对S和M片段的系统发育分析表明，萨马拉PUUV在俄罗斯伏尔加谱系中形成一个簇，明显不同于其他欧洲PUUV。使用IFA和酶联免疫吸附试验（ELISA），在来自萨马拉地区的7例HFRS患者和1例未确诊患者的血清中发现了抗PUUV抗体。这些数据表明，黄颈姬鼠是PUUV的主要天然宿主和传播媒介，而PUUV是萨马拉地区人类HFRS的主要病原体。
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