U.m.l Urgence Main Liban; Bellevue Medical Center; Mansourieh, Beirut Lebanon.
Organogenesis. 2009 Jul;5(3):127-33. doi: 10.4161/org.5.3.9584.
Nowadays, It is easy to define optimal conditions (cryoprotective agent, speed and steps of freezing, speed of warming) for the cryopreservation of a homogeneous cell population or a one cell-layer tissue. Meanwhile, It is still hard to obtain cryopreservation of composite organs. Each tissue has its own requirements and its own reactivity to the cryopreservation process. The challenge consists of, on the one hand, to select the ideal combination of cryoprotective agents that can fit the needs of the different tissues, and the definition of adequate technical parameters, on the other hand. All the experimental trials have studied the survival rate of non-vascularized cryopreserved tissues. The aim of our experimental work is to demonstrate the feasibility of cryopreserving a composite organ with its nutrient vessels "artery and veins" in order after thawing to revitalize it by reestablishing the blood irrigation by microsurgical vascular anastomosis. We report our experimental results on the cryopreservation of composite organs-amputated digits-xenotransplanted in the rabbit. Digital segments were cryopreserved, then revitalized after warming using vascular microsurgical techniques. Preliminary results are encouraging and may pave the way in the future to the microvascular allotransplantation of cryopreserved composite organs.
如今，对于同质细胞群体或单层组织的冷冻保存，很容易定义出最佳条件（保护剂、冷冻速度和步骤、复温速度）。然而，复合器官的冷冻保存仍然具有挑战性。每个组织都有自己的要求和对冷冻保存过程的反应。挑战在于一方面选择理想的冷冻保护剂组合，以满足不同组织的需求，另一方面定义适当的技术参数。所有的实验都研究了非血管化冷冻保存组织的存活率。我们的实验工作旨在证明冷冻保存具有营养血管（动脉和静脉）的复合器官的可行性，以便在解冻后通过显微血管吻合术重新建立血液灌流来恢复其活力。我们报告了在兔体内进行的复合器官-移植指节的冷冻保存实验结果。使用血管显微外科技术对指节进行冷冻保存，然后在复温后进行再血管化。初步结果令人鼓舞，可能为未来冷冻保存的复合器官的微血管同种异体移植铺平道路。
