Department of Mechanical Engineering; University of Minnesota; Minneapolis, MN USA.
Organogenesis. 2009 Jul;5(3):134-7. doi: 10.4161/org.5.3.9585.
Adult stem cells (hematopoietic and mesenchymal) have demonstrated tremendous human therapeutic potential. Currently, human embryonic stem cells are used principally for understanding development and disease progression but also hold tremendous therapeutic potential. The ability to preserve stem cells is critical for their use in clinical and research applications. Preservation of cells permits the transportation of cells between sites, as well as completion of safety and quality control testing. Preservation also permits the development of a 'manufacturing paradigm' for cell therapies, thereby maximizing the number of products that can be produced at a given facility. in this article, we will review modes of preservation and the current status of preservation of hematopoietic, mesenchymal and human embryonic stem cells. Current and emerging issues in the area of stem cell preservation will also be described.
成体干细胞（造血和间充质）已显示出巨大的人类治疗潜力。目前，主要使用人胚胎干细胞来了解发育和疾病进展，但也具有巨大的治疗潜力。保存干细胞的能力对于它们在临床和研究应用中的使用至关重要。细胞的保存允许细胞在不同地点之间运输，以及完成安全性和质量控制测试。保存还允许开发细胞治疗的“制造范例”，从而最大限度地增加给定设施可生产的产品数量。在本文中，我们将回顾保存模式以及目前保存造血、间充质和人胚胎干细胞的现状。还将描述干细胞保存领域当前和新出现的问题。
