Laha T K, Patnaik R K, Sen S
Cancer Chemotherapeutic Research Unit, College of Pharmaceutical Sciences, At/PO: Mohuda, Ganjam, Berhampur-760 002, India.
Indian J Pharm Sci. 2008 May-Jun;70(3):401-3. doi: 10.4103/0250-474X.43019.
A rapid and sensitive reverse phase high performance liquid chromatographic method is depicted for the qualitative and quantitative assay of letrozole in pharmaceutical dosage forms. Letrozole was chromatographed on a reverse phase C18 column with a mobile phase consisting of acetonitrile and phosphate buffer (pH 7.8) in the ratio of 70:30 v/v. The mobile phase was pumped at a flow rate of 1 ml/min. Acenaphthene was used as an internal standard and the eluents were monitored at 232 nm. The retention time of the drug was 3.385 min. With this method, linearity was observed in the range of 10-100 mug/ml. The LOD and LOQ were found to be 0.51 mug/ml and 1.52 mug/ml, respectively. The method was found to be applicable for analysis of drug in tablets. The results of the analysis were validated statistically.
描述了一种快速灵敏的反相高效液相色谱法，用于药物剂型中来曲唑的定性和定量测定。来曲唑在反相C18柱上进行色谱分析，流动相由乙腈和磷酸盐缓冲液（pH 7.8）按70:30 v/v的比例组成。流动相以1 ml/min的流速泵送。苊用作内标，洗脱液在232 nm处进行监测。药物的保留时间为3.385分钟。用该方法，在10-100μg/ml范围内观察到线性关系。检测限和定量限分别为0.51μg/ml和1.52μg/ml。该方法适用于片剂中药物的分析。分析结果经统计学验证。
