Gupta K R, Tajne M R, Wadodkar S G
Department of Pharmaceutical Sciences, RTM Nagpur University, Nagpur-33, India.
Indian J Pharm Sci. 2008 Jul-Aug;70(4):511-3. doi: 10.4103/0250-474X.44606.
The present work describes a two-wavelength method for simultaneous determination of metoprolol and hydrochlorthiazide in fixed dose combination tablet. The wavelengths selected for method were 257.8 nm, 282.9 nm and 315.0 nm. The absorbance difference at first two wavelengths was used for determination of metoprolol and the latter was used for determination of hydrochlorthiazide. The recovery value for the drugs from the tablet matrix was found to be 100.55% (metoprolol) and 99.97% (comparison with standard) and 98.09% (E1%, 1cm) for hydrochlorthiazide. The method has an advantage that hydrochlorthiazide can be estimated in combination, as there is no interference of metoprolol at 315.0 nm. The method was evaluated statistically for its accuracy and precision.
本研究描述了一种用于同时测定固定剂量复方片剂中酒石酸美托洛尔和氢氯噻嗪的双波长方法。该方法选择的波长为257.8nm、282.9nm和315.0nm。前两个波长处的吸光度差值用于测定酒石酸美托洛尔，后一个波长用于测定氢氯噻嗪。从片剂基质中回收的药物含量，酒石酸美托洛尔为100.55%（与标准品比较），氢氯噻嗪为98.09%（E1%，1cm）。该方法的优点是可以同时测定氢氯噻嗪，因为在315.0nm处酒石酸美托洛尔无干扰。对该方法的准确性和精密度进行了统计学评估。
