Rawool N D, Venkatchalam A
Department of Chemistry, Bhavan's College, Andheri (W), Mumbai-400 058, India.
Indian J Pharm Sci. 2011 Mar;73(2):219-23. doi: 10.4103/0250-474x.91565.
The present study deals with the estimation by RP-HPLC of two different drug components hydrochlorothiazide and metoprolol tartrate present in a tablet formulation. It is a simple, fast, precise and accurate high performance liquid chromatographic method. It is performed using phosphate buffer along with methanol as mobile phase, in the proportion of 60:40. The separation is done on a C(18) column and it is estimated at a λ(max) of 226 nm with a flow of 1 ml/min. The detection limits range from a 0.013 to 0.075 mg/ml for hydrochlorothiazide and 0.10 to 0.60 mg/ml for metoprolol tartrate, respectively. The specificity for interference of any peak with main peak of interest is checked. A scan of the individual drug was taken for assuring the λ(max). The system suitability by precision is also checked to ensure the analytical method. The method was found to be accurate and precise for estimation of the two drugs simultaneously.
本研究涉及采用反相高效液相色谱法（RP-HPLC）对片剂制剂中存在的两种不同药物成分氢氯噻嗪和酒石酸美托洛尔进行测定。这是一种简单、快速、精确且准确的高效液相色谱方法。使用磷酸盐缓冲液与甲醇按60:40的比例作为流动相进行测定。在C(18)柱上进行分离，并在226 nm的λ(max)处、流速为1 ml/min的条件下进行测定。氢氯噻嗪的检测限范围为0.013至0.075 mg/ml，酒石酸美托洛尔的检测限范围为0.10至0.60 mg/ml。检查了任何峰与目标主峰的干扰特异性。对每种药物进行扫描以确定λ(max)。还通过精密度检查系统适用性以确保分析方法。结果发现该方法对于同时测定这两种药物准确且精确。
