PREPARATIVE ISOLATION OF ISORHAMNETIN FROM STIGMA MAYDIS USING HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY.
作者信息
Cao Xiaoying, Wei Yun, Ito Yoichiro
机构信息
Applied Chemistry Department, Faculty of Sciences, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Chaoyang District, Beijing 100029, China.
出版信息
J Liq Chromatogr Relat Technol. 2009 Jan 1;32(2):273-280. doi: 10.1080/10826070802603369.
Abstract
Preparative high-speed countercurrent chromatography (HSCCC) has been successfully used for the isolation and purification of isorhamnetin from Stigma maydis. This was achieved in two stages: the first separation was performed with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (HEMW) at a volume ratio of 5:5:5:5, yielding isorhamnetinat 65.6 %, which is followed by the second run using a two-phase solvent system composed of HEMW 5:5:6:4, v/v. From 700 mg of the crude extract 11.8 mg of isorhamnetin was obtained at a high purity of 98%. The final identification was performed by MS, (1)H-NMR and (13)C-NMR spectra.
摘要
相似文献
1
PREPARATIVE ISOLATION OF ISORHAMNETIN FROM STIGMA MAYDIS USING HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY.
J Liq Chromatogr Relat Technol. 2009 Jan 1;32(2):273-280. doi: 10.1080/10826070802603369.
2
Preparative isolation and purification of flavonoids from the Chinese medicinal herb Belamcanda by high-speed countercurrent chromatography.
J Liq Chromatogr Relat Technol. 2009 Jan 1;32(16):2451-2461. doi: 10.1080/10826070903188245.
3
PREPARATIVE ISOLATION AND PURIFICATION OF FIVE FLAVONOIDS FROM POGOSTEMON CABLIN BENTH BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY AND PREPARATIVE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY.
J Liq Chromatogr Relat Technol. 2011 Jan 1;34(15):1617-1629. doi: 10.1080/10826076.2011.580486.
4
PREPARATIVE SEPARATION OF AXIFOLIN-3-GLUCOSIDE, HYPEROSIDE AND AMYGDALIN FROM PLANT EXTRACTS BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY.
J Liq Chromatogr Relat Technol. 2009 Jan 1;32(7):1010-1022. doi: 10.1080/10826070902790983.
5
[Preparation of cucurbitacin compounds in roots by high speed countercurrent chromatography].
Se Pu. 2022 Apr;40(4):364-371. doi: 10.3724/SP.J.1123.2021.07010.
6
Preparative separation of quercetin, ombuin and kaempferide from Gynostemma pentaphyllum by high-speed countercurrent chromatography.
J Chromatogr Sci. 2019 Mar 1;57(3):265-271. doi: 10.1093/chromsci/bmy110.
7
Preparative isolation and purification of psoralen and isopsoralen from Psoralea corylifolia by high-speed counter-current chromatography.
J Chromatogr A. 2004 Nov 19;1057(1-2):225-8. doi: 10.1016/j.chroma.2004.09.049.
8
Preparative separation of rhein from Chinese traditional herb by repeated high-speed counter-current chromatography.
J Chromatogr A. 2003 Oct 31;1017(1-2):125-30. doi: 10.1016/j.chroma.2003.08.015.
9
Preparative separation and purification of sulforaphene from radish seeds by high-speed countercurrent chromatography.
Food Chem. 2013 Jan 15;136(2):309-15. doi: 10.1016/j.foodchem.2012.08.042. Epub 2012 Aug 30.
10
Preparative isolation and purification of theaflavins and catechins by high-speed countercurrent chromatography.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 May 15;867(2):282-6. doi: 10.1016/j.jchromb.2008.04.005. Epub 2008 Apr 9.
引用本文的文献
1
Anti-inflammatory and cytotoxic assessment of flavonoids isolated from Viola odorata flowers with computer-guided docking study.
Sci Rep. 2025 Sep 18;15(1):32602. doi: 10.1038/s41598-025-19442-4.
2
Exploring the Therapeutic Value of Some Vegetative Parts of and : A Literature Review on Bioactive Profiles and Their Pharmaceutical and Cosmetic Interest.
Molecules. 2025 Jul 26;30(15):3144. doi: 10.3390/molecules30153144.
3
In Vitro Prebiotic Potential of Leaf Extract and Its Active Constituent.
Molecules. 2025 Jul 25;30(15):3124. doi: 10.3390/molecules30153124.
4
Therapeutic potential of isolated flavonoids from Anise and coriander aerial parts in antimicrobial efficacy, molecular docking, ADMET, and dynamic simulations.
Sci Rep. 2025 Jul 21;15(1):26485. doi: 10.1038/s41598-025-10927-w.
5
Metabolomics analyses and comparative insight to neuroprotective potential of unripe fruits and leaves of Citrus aurantium ethanolic extracts against cadmium-induced rat brain dysfunction: involvement of oxidative stress and akt-mediated CREB/BDNF and GSK3β/NF-κB signaling pathways.
Metab Brain Dis. 2025 Jan 6;40(1):89. doi: 10.1007/s11011-024-01513-6.
6
Molecular Insights Into β-Glucuronidase Inhibition by Alhagi Graecorum Flavonoids: A Computational and Experimental Approach.
ChemistryOpen. 2025 Mar;14(3):e202400325. doi: 10.1002/open.202400325. Epub 2024 Nov 19.
7
Quercetin: A Functional Food-Flavonoid Incredibly Attenuates Emerging and Re-Emerging Viral Infections through Immunomodulatory Actions.
Molecules. 2023 Jan 17;28(3):938. doi: 10.3390/molecules28030938.
8
Absorption Coefficients of Phenolic Structures in Different Solvents Routinely Used for Experiments.
Molecules. 2021 Jul 31;26(15):4656. doi: 10.3390/molecules26154656.
9
Thalassosterol, a New Cytotoxic Aromatase Inhibitor Ergosterol Derivative from the Red Sea Seagrass .
Mar Drugs. 2020 Jul 8;18(7):354. doi: 10.3390/md18070354.
10
Sedative and Anxiolytic Activities of (L.) Mill.: An Experimental Assessment in Mice.
Molecules. 2020 Apr 16;25(8):1844. doi: 10.3390/molecules25081844.
本文引用的文献
1
[Study on the chemical constituents of flavones from corn silk].
Zhong Yao Cai. 2007 Feb;30(2):164-6.
2
Large-scale isolation of flavan-3-ol phloroglucinol adducts by high-speed counter-current chromatography.
J Chromatogr A. 2005 Apr 29;1072(2):217-22. doi: 10.1016/j.chroma.2005.03.025.
3
Isolation and purification of the bioactive carotenoid zeaxanthin from the microalga Microcystis aeruginosa by high-speed counter-current chromatography.
J Chromatogr A. 2005 Feb 4;1064(2):183-6. doi: 10.1016/j.chroma.2004.12.065.
Zotero 插件