Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt.
Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, Egypt.
Mar Drugs. 2020 Jul 8;18(7):354. doi: 10.3390/md18070354.
(Forssk.) Den Hartog is a seagrass belonging to the plant family Cymodoceaceae with ubiquitous phytoconstituents and important pharmacological potential, including antioxidant, antiviral, and cytotoxic activities. In this work, a new ergosterol derivative named thalassosterol () was isolated from the methanolic extract of growing in the Red Sea, along with two known first-reported sterols, namely ergosterol () and stigmasterol (), using different chromatographic techniques. The structure of the new compound was established based on 1D and 2D NMR spectroscopy and high-resolution mass spectrometry (HR-MS) and by comparison with the literature data. The new ergosterol derivative showed significant in vitro antiproliferative potential against the human cervical cancer cell line (HeLa) and human breast cancer (MCF-7) cell lines, with IC values of 8.12 and 14.24 µM, respectively. In addition, docking studies on the new sterol explained the possible binding interactions with an aromatase enzyme; this inhibition is beneficial in both cervical and breast cancer therapy. A metabolic analysis of the crude extract of using liquid chromatography combined with high-resolution electrospray ionization mass spectrometry (LC-ESI-HR-MS) revealed the presence of an array of phenolic compounds, sterols and ceramides, as well as di- and triglycerides.
(Forssk.) Den Hartog 是一种属于水鳖科的海草，具有广泛存在的植物化学成分和重要的药理学潜力，包括抗氧化、抗病毒和细胞毒性活性。在这项工作中，从生长在红海的中分离到一种新的麦角甾醇衍生物，命名为塔拉萨甾醇（），以及两种已知的首次报道的甾醇，即麦角甾醇（）和豆甾醇（），使用了不同的色谱技术。根据 1D 和 2D NMR 光谱和高分辨率质谱（HR-MS）以及与文献数据的比较，确定了新化合物的结构。新的麦角甾醇衍生物对人宫颈癌（HeLa）和人乳腺癌（MCF-7）细胞系表现出显著的体外抗增殖活性，IC 值分别为 8.12 和 14.24 µM。此外，对新甾体的对接研究解释了与芳香酶酶的可能结合相互作用；这种抑制在宫颈癌和乳腺癌的治疗中都是有益的。使用液相色谱结合高分辨率电喷雾电离质谱（LC-ESI-HR-MS）对粗提取物进行代谢分析，揭示了存在一系列酚类化合物、甾醇和神经酰胺，以及二酯和三酯。
