嗜热四膜虫大亚基核糖体RNA基因的突变赋予其茴香霉素抗性和冷敏感性。
A mutation in the large subunit ribosomal RNA gene of Tetrahymena confers anisomycin resistance and cold sensitivity.
作者信息
Sweeney R, Yao C H, Yao M C
机构信息
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
出版信息
Genetics. 1991 Feb;127(2):327-34. doi: 10.1093/genetics/127.2.327.
Abstract
Anisomycin, an antibiotic that specifically inhibits the peptidyl transfer function of eukaryotic ribosomes, has been used to select resistant mutants in Tetrahymena thermophila. A mutation conferring anisomycin resistance (an-r) has been localized to a 1.2-kb fragment of the large subunit ribosomal RNA (rRNA) gene by transformation via microinjection. A single base pair change was detected within this region. Nine independently isolated an-r mutants had the same base pair change. T. thermophila strains that are homozygous for this mutation are cold sensitive, unable to mate and grossly abnormal in cell morphology.
摘要
茴香霉素是一种特异性抑制真核生物核糖体肽基转移功能的抗生素，已被用于在嗜热四膜虫中筛选抗性突变体。通过显微注射转化，赋予茴香霉素抗性（an-r）的突变已定位到大亚基核糖体RNA（rRNA）基因的一个1.2 kb片段上。在该区域内检测到一个单碱基对变化。九个独立分离的an-r突变体具有相同的碱基对变化。对于该突变纯合的嗜热四膜虫菌株对冷敏感，无法交配，并且细胞形态严重异常。
相似文献
1
A mutation in the large subunit ribosomal RNA gene of Tetrahymena confers anisomycin resistance and cold sensitivity.嗜热四膜虫大亚基核糖体RNA基因的突变赋予其茴香霉素抗性和冷敏感性。
Genetics. 1991 Feb;127(2):327-34. doi: 10.1093/genetics/127.2.327.
2
An intragenic suppressor of cold sensitivity identifies potentially interacting bases in the peptidyl transferase center of Tetrahymena rRNA.一个冷敏感性的基因内抑制子鉴定出了嗜热四膜虫核糖体RNA肽基转移酶中心中潜在相互作用的碱基。
Genetics. 1998 Jun;149(2):937-46. doi: 10.1093/genetics/149.2.937.
3
4
Transformation of Tetrahymena thermophila by microinjection of ribosomal RNA genes.通过显微注射核糖体RNA基因对嗜热四膜虫进行转化。
Proc Natl Acad Sci U S A. 1986 Jun;83(12):4369-73. doi: 10.1073/pnas.83.12.4369.
5
Circular ribosomal DNA plasmids transform Tetrahymena thermophila by homologous recombination with endogenous macronuclear ribosomal DNA.环状核糖体DNA质粒通过与内源性大核核糖体DNA的同源重组转化嗜热四膜虫。
Proc Natl Acad Sci U S A. 1988 Jul;85(14):5151-5. doi: 10.1073/pnas.85.14.5151.
6
Transformation of Tetrahymena thermophila by electroporation and parameters effecting cell survival.嗜热四膜虫的电穿孔转化及影响细胞存活的参数
Exp Cell Res. 1988 Feb;174(2):525-32. doi: 10.1016/0014-4827(88)90322-9.
7
Paromomycin resistance in Leishmania tropica: lack of correlation with mutation in the small subunit ribosomal RNA gene.热带利什曼原虫对巴龙霉素的耐药性：与核糖体RNA基因小亚基突变缺乏相关性
Am J Trop Med Hyg. 1994 Dec;51(6):758-66. doi: 10.4269/ajtmh.1994.51.758.
8
23S ribosomal RNA mutations in halobacteria conferring resistance to the anti-80S ribosome targeted antibiotic anisomycin.嗜盐菌中赋予对靶向80S核糖体的抗生素茴香霉素抗性的23S核糖体RNA突变。
Nucleic Acids Res. 1987 Mar 25;15(6):2431-43. doi: 10.1093/nar/15.6.2431.
9
Regulation of ribosome phosphorylation and antibiotic sensitivity in Tetrahymena thermophila: A correlation.嗜热四膜虫核糖体磷酸化与抗生素敏感性的调控：一种相关性
Cell. 1981 Oct;26(1 Pt 1):47-56. doi: 10.1016/0092-8674(81)90032-5.
10
A drug-resistant mutation in the ribosomal DNA of Tetrahymena.
Proc Natl Acad Sci U S A. 1985 May;82(9):2844-6. doi: 10.1073/pnas.82.9.2844.
引用本文的文献
1
Azole resistance in a mutant lacking the ABC transporter depends on TOR signaling.唑类耐药突变体中 ABC 转运蛋白缺失依赖于 TOR 信号。
J Biol Chem. 2018 Jan 12;293(2):412-432. doi: 10.1074/jbc.M117.807032. Epub 2017 Nov 20.
2
rRNA mutants in the yeast peptidyltransferase center reveal allosteric information networks and mechanisms of drug resistance.酵母肽基转移酶中心的rRNA突变体揭示了变构信息网络和耐药机制。
Nucleic Acids Res. 2008 Mar;36(5):1497-507. doi: 10.1093/nar/gkm1179. Epub 2008 Jan 18.
3
Isolation of antibiotic resistance mutations in the rRNA by using an in vitro selection system.利用体外筛选系统分离rRNA中的抗生素抗性突变
Proc Natl Acad Sci U S A. 2004 Mar 16;101(11):3786-91. doi: 10.1073/pnas.0307596101. Epub 2004 Mar 4.
4
mkp-1 encoding mitogen-activated protein kinase phosphatase 1, a verotoxin 1 responsive gene, detected by differential display reverse transcription-PCR in Caco-2 cells.mkp-1编码丝裂原活化蛋白激酶磷酸酶1，一种由差异显示逆转录聚合酶链反应在Caco-2细胞中检测到的维罗毒素1反应基因。
Infect Immun. 2000 May;68(5):2791-6. doi: 10.1128/IAI.68.5.2791-2796.2000.
5
Expanded versions of the 16S and 23S ribosomal RNA mutation databases (16SMDBexp and 23SMDBexp).16S和23S核糖体RNA突变数据库的扩展版本（16SMDBexp和23SMDBexp）。
Nucleic Acids Res. 1998 Jan 1;26(1):280-4. doi: 10.1093/nar/26.1.280.
6
Regulatory sequences for the amplification and replication of the ribosomal DNA minichromosome in Tetrahymena thermophila.嗜热四膜虫核糖体DNA微型染色体扩增与复制的调控序列。
Mol Cell Biol. 1997 Dec;17(12):7237-47. doi: 10.1128/MCB.17.12.7237.
7
Ribotoxic stress response: activation of the stress-activated protein kinase JNK1 by inhibitors of the peptidyl transferase reaction and by sequence-specific RNA damage to the alpha-sarcin/ricin loop in the 28S rRNA.核糖体毒性应激反应：肽基转移酶反应抑制剂以及28S rRNA中α-肌动蛋白/蓖麻毒素环的序列特异性RNA损伤激活应激激活蛋白激酶JNK1 。
Mol Cell Biol. 1997 Jun;17(6):3373-81. doi: 10.1128/MCB.17.6.3373.
8
Mutations in an essential U2 small nuclear RNA structure cause cold-sensitive U2 small nuclear ribonucleoprotein function by favoring competing alternative U2 RNA structures.
Mol Cell Biol. 1994 Mar;14(3):1689-97. doi: 10.1128/mcb.14.3.1689-1697.1994.
9
A spontaneous point mutation in the single 23S rRNA gene of the thermophilic arachaeon Sulfolobus acidocaldarius confers multiple drug resistance.嗜热古菌嗜酸热硫化叶菌（Sulfolobus acidocaldarius）单一的23S rRNA基因中的一个自发点突变赋予了多重耐药性。
J Bacteriol. 1994 Dec;176(24):7744-7. doi: 10.1128/jb.176.24.7744-7747.1994.
10
Erythromycin resistance by ribosome modification.通过核糖体修饰产生的红霉素耐药性。
Antimicrob Agents Chemother. 1995 Mar;39(3):577-85. doi: 10.1128/AAC.39.3.577.
本文引用的文献
1
Replication of the extrachromosomal ribosomal RNA genes of Tetrahymena thermophilia.嗜热四膜虫染色体外核糖体RNA基因的复制
Nucleic Acids Res. 1981 Jul 24;9(14):3531-43. doi: 10.1093/nar/9.14.3531.
2
Cloning of yeast gene for trichodermin resistance and ribosomal protein L3.抗木霉菌素酵母基因与核糖体蛋白L3的克隆
Proc Natl Acad Sci U S A. 1981 Jan;78(1):238-42. doi: 10.1073/pnas.78.1.238.
3
Allele-specific, selective amplification of a ribosomal RNA gene in Tetrahymena thermophila.嗜热四膜虫核糖体RNA基因的等位基因特异性选择性扩增。
Cell. 1982 Mar;28(3):595-604. doi: 10.1016/0092-8674(82)90214-8.
4
Different nucleotide changes in the large rRNA gene of the mitochondrial DNA confer chloramphenicol resistance on two human cell lines.线粒体DNA的大核糖体RNA基因中的不同核苷酸变化赋予了两种人类细胞系氯霉素抗性。
Nucleic Acids Res. 1981 Nov 11;9(21):5785-95. doi: 10.1093/nar/9.21.5785.
5
Structure of ribosomal RNA.核糖体RNA的结构。
Annu Rev Biochem. 1984;53:119-62. doi: 10.1146/annurev.bi.53.070184.001003.
6
Cytogenetics of genomic exclusion in Tetrahymena.四膜虫基因组排除的细胞遗传学
Genetics. 1967 Apr;55(4):797-822. doi: 10.1093/genetics/55.4.797.
7
Studies on nuclear structure and function in Tetrahymena pyriformis. II. Isolation of macro- and micronuclei.梨形四膜虫细胞核结构与功能的研究。II. 大核和小核的分离。
J Cell Biol. 1970 Dec;47(3):619-30. doi: 10.1083/jcb.47.3.619.
8
9
23S ribosomal RNA mutations in halobacteria conferring resistance to the anti-80S ribosome targeted antibiotic anisomycin.嗜盐菌中赋予对靶向80S核糖体的抗生素茴香霉素抗性的23S核糖体RNA突变。
Nucleic Acids Res. 1987 Mar 25;15(6):2431-43. doi: 10.1093/nar/15.6.2431.
10
Transformation of Tetrahymena thermophila by microinjection of ribosomal RNA genes.通过显微注射核糖体RNA基因对嗜热四膜虫进行转化。
Proc Natl Acad Sci U S A. 1986 Jun;83(12):4369-73. doi: 10.1073/pnas.83.12.4369.
Zotero 插件