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嗜热四膜虫大亚基核糖体RNA基因的突变赋予其茴香霉素抗性和冷敏感性。

A mutation in the large subunit ribosomal RNA gene of Tetrahymena confers anisomycin resistance and cold sensitivity.

作者信息

Sweeney R, Yao C H, Yao M C

机构信息

Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.

出版信息

Genetics. 1991 Feb;127(2):327-34. doi: 10.1093/genetics/127.2.327.

DOI:10.1093/genetics/127.2.327
PMID:2004706
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1204360/
Abstract

Anisomycin, an antibiotic that specifically inhibits the peptidyl transfer function of eukaryotic ribosomes, has been used to select resistant mutants in Tetrahymena thermophila. A mutation conferring anisomycin resistance (an-r) has been localized to a 1.2-kb fragment of the large subunit ribosomal RNA (rRNA) gene by transformation via microinjection. A single base pair change was detected within this region. Nine independently isolated an-r mutants had the same base pair change. T. thermophila strains that are homozygous for this mutation are cold sensitive, unable to mate and grossly abnormal in cell morphology.

摘要

茴香霉素是一种特异性抑制真核生物核糖体肽基转移功能的抗生素，已被用于在嗜热四膜虫中筛选抗性突变体。通过显微注射转化，赋予茴香霉素抗性（an-r）的突变已定位到大亚基核糖体RNA（rRNA）基因的一个1.2 kb片段上。在该区域内检测到一个单碱基对变化。九个独立分离的an-r突变体具有相同的碱基对变化。对于该突变纯合的嗜热四膜虫菌株对冷敏感，无法交配，并且细胞形态严重异常。

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A mutation in the large subunit ribosomal RNA gene of Tetrahymena confers anisomycin resistance and cold sensitivity.嗜热四膜虫大亚基核糖体RNA基因的突变赋予其茴香霉素抗性和冷敏感性。
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本文引用的文献

1
Replication of the extrachromosomal ribosomal RNA genes of Tetrahymena thermophilia.嗜热四膜虫染色体外核糖体RNA基因的复制
Nucleic Acids Res. 1981 Jul 24;9(14):3531-43. doi: 10.1093/nar/9.14.3531.
2
Cloning of yeast gene for trichodermin resistance and ribosomal protein L3.抗木霉菌素酵母基因与核糖体蛋白L3的克隆
Proc Natl Acad Sci U S A. 1981 Jan;78(1):238-42. doi: 10.1073/pnas.78.1.238.
3
Allele-specific, selective amplification of a ribosomal RNA gene in Tetrahymena thermophila.嗜热四膜虫核糖体RNA基因的等位基因特异性选择性扩增。
Cell. 1982 Mar;28(3):595-604. doi: 10.1016/0092-8674(82)90214-8.
4
Different nucleotide changes in the large rRNA gene of the mitochondrial DNA confer chloramphenicol resistance on two human cell lines.线粒体DNA的大核糖体RNA基因中的不同核苷酸变化赋予了两种人类细胞系氯霉素抗性。
Nucleic Acids Res. 1981 Nov 11;9(21):5785-95. doi: 10.1093/nar/9.21.5785.
5
Structure of ribosomal RNA.核糖体RNA的结构。
Annu Rev Biochem. 1984;53:119-62. doi: 10.1146/annurev.bi.53.070184.001003.
6
Cytogenetics of genomic exclusion in Tetrahymena.四膜虫基因组排除的细胞遗传学
Genetics. 1967 Apr;55(4):797-822. doi: 10.1093/genetics/55.4.797.
7
Studies on nuclear structure and function in Tetrahymena pyriformis. II. Isolation of macro- and micronuclei.梨形四膜虫细胞核结构与功能的研究。II. 大核和小核的分离。
J Cell Biol. 1970 Dec;47(3):619-30. doi: 10.1083/jcb.47.3.619.
8
The nucleotide sequence of the 17S ribosomal RNA gene of Tetrahymena thermophila and the identification of point mutations resulting in resistance to the antibiotics paromomycin and hygromycin.嗜热四膜虫17S核糖体RNA基因的核苷酸序列以及导致对抗生素巴龙霉素和潮霉素产生抗性的点突变的鉴定。
J Biol Chem. 1985 May 25;260(10):6334-40.
9
23S ribosomal RNA mutations in halobacteria conferring resistance to the anti-80S ribosome targeted antibiotic anisomycin.嗜盐菌中赋予对靶向80S核糖体的抗生素茴香霉素抗性的23S核糖体RNA突变。
Nucleic Acids Res. 1987 Mar 25;15(6):2431-43. doi: 10.1093/nar/15.6.2431.
10
Transformation of Tetrahymena thermophila by microinjection of ribosomal RNA genes.通过显微注射核糖体RNA基因对嗜热四膜虫进行转化。
Proc Natl Acad Sci U S A. 1986 Jun;83(12):4369-73. doi: 10.1073/pnas.83.12.4369.