Department of Pharmacology, Faculty of Health Sciences, University of Pretoria, PO Box 2034, Pretoria 0001, South Africa.
Inflammopharmacology. 2010 Feb;18(1):33-9. doi: 10.1007/s10787-009-0026-8. Epub 2010 Jan 3.
It has been shown in a previous study that brown coal-derived potassium humate is safe and effective in suppressing contact hypersensitivity in rats. In this study the efficacy of potassium humate on other types of inflammation was determined. Preparative TLC followed by mass spectroscopy was used in an attempt to fingerprint the product. The effects of potassium humate, at an oral dose of 60 mg/kg bodyweight, on a delayed type hypersensitivity reaction, a carrageenan-induced inflammation model and an allogeneic graft-versus-host reaction (GVHR) in rats were investigated. Paw oedema was used as a measure of inflammation. It was found that potassium humate had no effect on the delayed type hypersensitivity reaction but significantly inhibited the increase in paw volume of the carrageenan-induced oedema in rats which compared favourably with indomethacin treatment. Furthermore, potassium humate inhibited the GVHR induced in normal and cyclophosphamide-treated immune-incompetent rats. The identification of a naturally occurring compound that is safe and effective in reducing different types of inflammation merits further evaluation in clinical trials.
先前的研究表明，褐煤衍生的腐植酸钾在抑制大鼠接触性过敏方面是安全有效的。本研究旨在确定腐植酸钾对其他类型炎症的疗效。采用制备性 TLC 结合质谱技术对该产品进行了指纹图谱分析。研究了腐植酸钾（口服剂量为 60mg/kg 体重）对大鼠迟发型超敏反应、角叉菜胶诱导的炎症模型和同种异体移植物抗宿主反应（GVHR）的影响。爪肿胀被用作炎症的衡量指标。结果发现，腐植酸钾对迟发型超敏反应没有影响，但能显著抑制角叉菜胶诱导的大鼠爪肿胀，其效果与吲哚美辛相当。此外，腐植酸钾还抑制了正常和环磷酰胺处理的免疫无能大鼠的 GVHR。这种天然存在的化合物在减少不同类型炎症方面安全有效，值得在临床试验中进一步评估。
