Shi Wei, Yao Jian, Chen Xue, Lin Weiwei, Gu Xiaosong, Wang Xiaodong
Department of Histology and Embryology, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, Jiangsu, People's Republic of China.
Artif Cells Blood Substit Immobil Biotechnol. 2010;38(1):29-37. doi: 10.3109/10731190903495751.
The purpose of this study was to evaluate the feasibility of using tissue-engineered nerve grafts for delayed repair of peripheral nerve defects. A 1-month delayed, 10-mm long sciatic nerve defect was created for rats, which were divided into three grafted groups and a non-grafted group. For bridging the nerve defects, the rats in three grafted groups were subjected to surgical repair with tissue-engineered nerve grafts made of a chitosan/polyglycolic acid (PGA) conduit filled with neural stem cells (NSCs), chitosan/PGA conduits, and autologous nerve grafts, respectively. At 3 months after nerve grafting, the data from electrophysiology, retrograde tracing and histological investigation revealed that the better outcomes in sciatic nerve regeneration and target muscle re-innervation were achieved in three grafted groups as compared to those in non-grafted group without major differences between three grafted groups. Our results suggest that grafting of chitosan/PGA conduits might be a promising technique for repairing peripheral nerve injuries after a 1-month delay, while introduction of NSCs seem to show no significant additional benefits to regenerative outcomes.
本研究的目的是评估使用组织工程神经移植物延迟修复周围神经缺损的可行性。为大鼠制造了一个1个月延迟、10毫米长的坐骨神经缺损，并将其分为三个移植组和一个非移植组。为了桥接神经缺损，三个移植组的大鼠分别接受了由填充神经干细胞（NSCs）的壳聚糖/聚乙醇酸（PGA）导管、壳聚糖/PGA导管和自体神经移植物制成的组织工程神经移植物进行手术修复。在神经移植后3个月，电生理学、逆行示踪和组织学研究的数据显示，与非移植组相比，三个移植组在坐骨神经再生和靶肌肉再支配方面取得了更好的结果，三个移植组之间没有显著差异。我们的结果表明，壳聚糖/PGA导管移植可能是一种有前景的技术，用于延迟1个月后修复周围神经损伤，而引入神经干细胞似乎对再生结果没有显著的额外益处。
