通过氮固定条件下诱导的 Frankia 基因抑制性消减杂交鉴定。
Identification by suppression subtractive hybridization of Frankia genes induced under nitrogen-fixing conditions.
机构信息
Graduate School of Science and Engineering, Kagoshima University, Korimoto 1-21-35, Kagoshima 890-0065, Japan.
出版信息
Appl Environ Microbiol. 2010 Mar;76(5):1692-4. doi: 10.1128/AEM.01813-09. Epub 2010 Jan 4.
Abstract
Frankia is an actinobacterium that fixes nitrogen under both symbiotic and free-living conditions. We identified genes upregulated in free-living nitrogen-fixing cells by using suppression subtractive hybridization. They included genes with predicted functions related to nitrogen fixation, as well as with unknown function. Their upregulation was a novel finding in Frankia.
摘要
弗兰克氏菌是一种放线菌，能够在共生和自由生活条件下固定氮。我们通过抑制消减杂交技术鉴定了自由生活固氮细胞中上调的基因。它们包括具有预测固氮功能以及未知功能的基因。它们的上调在弗兰克氏菌中是一个新的发现。
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