Sorokine I, Ben-Mahrez K, Bracone A, Thierry D, Ishii S, Imamoto F, Kohiyama M
Institut Jacques Monod, Université Paris VII, France.
Int J Cancer. 1991 Mar 12;47(5):665-9. doi: 10.1002/ijc.2910470506.
Bacterially expressed mouse c-myb protein was purified from E. coli extracts and used as antigen to screen human sera for circulating anti-c-myb oncogene product antibodies. Using Western blotting, we have found that human sera contain IgG antibodies to the c-myb gene product. The percentage of positive sera in cancer patients appears to be dependent upon cancer type and is significantly higher in breast-cancer patients than in normal donors: 43% of sera from breast-cancer patients are positive, whereas in neuroblastoma cancer patients the production of IgG anti-c-myb appears to be rare. No significant correlation was observed between the presence of circulating anti-c-myb antibodies and the expression of the c-myb gene in breast tumors.
从大肠杆菌提取物中纯化出细菌表达的小鼠c-myb蛋白，并将其用作抗原，以筛选人血清中循环的抗c-myb癌基因产物抗体。通过蛋白质印迹法，我们发现人血清中含有针对c-myb基因产物的IgG抗体。癌症患者中阳性血清的百分比似乎取决于癌症类型，乳腺癌患者中的阳性率明显高于正常供体：43%的乳腺癌患者血清呈阳性，而神经母细胞瘤患者中IgG抗c-myb的产生似乎很少见。在乳腺癌中，循环抗c-myb抗体的存在与c-myb基因的表达之间未观察到显著相关性。
