Ben-Mahrez K, Thierry D, Sorokine I, Danna-Muller A, Kohiyama M
Institut Jacques Monod, Université Paris VII, France.
Br J Cancer. 1988 Jun;57(6):529-34. doi: 10.1038/bjc.1988.123.
We have partially purified an archaebacterial protein of 84 kD which shares common epitopes with the human c-myc protein as shown by its cross-reactivity with a commercialized anti-human c-myc antiserum. An antiserum raised against the 84 kD protein recognizes a 60 kD protein from HL-60 nuclei. This protein is also recognized by the anti-human c-myc antiserum. Using this archaebacterial protein as antigen for Western blot analysis, we found that the human c-myc oncogene product could be immunogenic and that it is possible, in some spontaneously occurring human tumours, to detect antibodies against the c-myc gene product in the serum of cancer patients.
我们已经部分纯化了一种84 kD的古细菌蛋白，该蛋白与人类c-myc蛋白具有共同的表位，这通过它与商业化的抗人c-myc抗血清的交叉反应得以证明。针对84 kD蛋白产生的抗血清识别来自HL-60细胞核的一种60 kD蛋白。这种蛋白也能被抗人c-myc抗血清识别。用这种古细菌蛋白作为抗原进行蛋白质印迹分析，我们发现人类c-myc癌基因产物可能具有免疫原性，并且在一些自发发生的人类肿瘤中，有可能在癌症患者的血清中检测到针对c-myc基因产物的抗体。
