Institute of Rheumatology, Tokyo Women's Medical University, Shinjyuku-ku, Tokyo, Japan.
Rheumatol Int. 2011 May;31(5):617-22. doi: 10.1007/s00296-009-1336-2. Epub 2010 Jan 5.
The anti-cyclic citrullinated peptide (CCP) antibody has become increasingly important in the diagnosis of rheumatoid arthritis (RA), especially for early diagnosis. The purpose of this study is to compare the diagnostic usefulness of the second and third generation anti-CCP antibody kits among Japanese patients with RA. Anti-CCP antibody titers were measured with the second generation (MESACUP CCP test, Medical and biological laboratories) and third generation (QUANTA Lite CCP3 IgG ELISA, Inova Diagnostics) kits using serum samples from 106 rheumatoid arthritis (RA) patients and 57 non-RA patients. Sensitivities and specificities were compared. The sensitivity and specificity of the second generation anti-CCP (anti-CCP2) kit were 88.7 and 89.5%, and those of the third generation anti-CCP (anti-CCP3) kit were 91.5 and 87.7%. Area under the receiver operating curve showed that anti-CCP2 and anti-CCP3 had similar diagnostic performances. Diagnostic performance of the anti-CCP3 assay was comparable with the anti-CCP2 assay in Japanese patients with RA.
抗环瓜氨酸肽（CCP）抗体在类风湿关节炎（RA）的诊断中变得越来越重要，特别是在早期诊断中。本研究旨在比较第二代和第三代抗 CCP 抗体试剂盒在日本 RA 患者中的诊断效用。使用来自 106 例类风湿关节炎（RA）患者和 57 例非 RA 患者的血清样本，用第二代（MESACUP CCP 试验，Medical and biological laboratories）和第三代（QUANTA Lite CCP3 IgG ELISA，Inova Diagnostics）试剂盒测量抗 CCP 抗体滴度。比较了敏感性和特异性。第二代抗 CCP（抗 CCP2）试剂盒的敏感性和特异性分别为 88.7%和 89.5%，第三代抗 CCP（抗 CCP3）试剂盒的敏感性和特异性分别为 91.5%和 87.7%。受试者工作特征曲线下面积表明抗 CCP2 和抗 CCP3 具有相似的诊断性能。抗 CCP3 检测在日本 RA 患者中的诊断性能与抗 CCP2 检测相当。
