Laboratory for Biomedical Microsystems, Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA.
IEEE Trans Nanobioscience. 2009 Sep;8(3):226-36. doi: 10.1109/TNB.2009.2025131.
1-D magnetic nanowires provide a powerful tool for investigating biological systems because such nanomaterials possess unique magnetic properties, which allow effective manipulation of cellular and subcellular objects. In this study, we report the rotational maneuver of ferromagnetic nanowires and their applications in cell manipulation. The rotational maneuver is studied under two different suspension conditions. The rotation of nanowires in the fluid is analyzed using Stokes flow assumption. Experimental results show that when the nanowires develop contacts with the bottom surfaces, the rotational maneuver under a modest external magnetic field can generate rapid lateral motion. The floating nanowires, on the other hand, do not exhibit substantial lateral displacements. Cell manipulation using skeletal myoblasts C2C12 shows that living cells can be manipulated efficiently on the bottom surface by the rotational maneuver of the attached nanowires. We also demonstrate the use of rotational maneuver of nanowires for creating 3-D nanowire clusters and multicellular clusters. This study is expected to add to the knowledge of nanowire-based cell manipulation and contribute to a full spectrum of control strategies for efficient use of nanowires for micro-total-analysis. It may also facilitate mechanobiological studies at cellular level, and provide useful insights for development of 3-D in vivo-like multicellular models for various applications in tissue engineering.
1-D 磁纳米线为研究生物系统提供了有力的工具，因为这些纳米材料具有独特的磁性能，可以有效地操纵细胞和亚细胞物体。在这项研究中，我们报告了铁磁纳米线的旋转操作及其在细胞操纵中的应用。在两种不同的悬浮条件下研究了旋转操作。使用 Stokes 流假设分析了纳米线在流体中的旋转。实验结果表明，当纳米线与底部表面接触时，适度的外磁场下的旋转操作可以产生快速的横向运动。另一方面，悬浮的纳米线不会发生明显的横向位移。使用骨骼肌成肌细胞 C2C12 进行细胞操纵表明，附着的纳米线的旋转操作可以有效地在底部表面操纵活细胞。我们还展示了使用纳米线的旋转操作来创建 3-D 纳米线簇和多细胞簇。这项研究有望增加基于纳米线的细胞操纵的知识，并为微全分析的有效利用纳米线的各种控制策略做出贡献。它还可以促进细胞水平的机械生物学研究，并为各种组织工程应用的 3-D 类似体内多细胞模型的开发提供有用的见解。
